The role of transforming growth factor beta in myopia development

被引：5
作者：
Ku, Hsiangyu [1 ,2 ,3 ]
Chen, Jamie Jiin-Yi [4 ]
Chen, Wei [1 ,2 ]
Tien, Peng-Tai [4 ,5 ]
Lin, Hui-Ju [4 ,6 ]
Wan, Lei [6 ,7 ,8 ,9 ]
Xu, Gezhi [1 ,2 ,10 ]
机构：
[1] Fudan Univ, Eye & ENT Hosp, Shanghai Med Coll, Dept Ophthalmol & Visual Sci, Shanghai 200031, Peoples R China
[2] Shanghai Key Lab Visual Impairment & Restorat, Shanghai 200031, Peoples R China
[3] Yunnan Univ, Dept Pediat Ophthalmol, Affiliated Hosp, Kunming, Peoples R China
[4] China Med Univ Hosp, Eye Ctr, Taichung, Taiwan
[5] China Med Univ, Grad Inst Clin Med Sci, Coll Med, Taichung, Taiwan
[6] China Med Univ, Sch Chinese Med, Taichung, Taiwan
[7] Asia Univ, Dept Med Lab Sci & Biotechnol, Taichung, Taiwan
[8] China Med Univ Hosp, Dept Obstet & Gynecol, Taichung, Taiwan
[9] 91,Hsueh Shih Rd, Taichung 40402, Taiwan
[10] 83,Fen Yang Rd, Shanghai 200031, Peoples R China
来源：
MOLECULAR IMMUNOLOGY | 2024年 / 167卷
关键词：
Myopia; Transforming growth factor beta; Inflammation; Tree shrew; Retinal signaling; NF-KAPPA-B; ENDOTOXIN-INDUCED UVEITIS; FORM-DEPRIVATION MYOPIA; TGF-BETA; GENE-EXPRESSION; TREE SHREW; CROSS-TALK; ACTIVATION; SMAD7; TGF-BETA-2;
D O I：
10.1016/j.molimm.2024.01.011
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Myopia is widely recognized as an epidemic. Studies have found a link between Transforming Growth Factorbeta (TGF-beta) and myopia, but the specific molecular mechanisms are not fully understood. In this study, a monocular model in tree shrews (Tupaia belangeri) was established to verify the molecular mechanism of TGF-beta in myopia. The results indicated that there were significant changes in TGF-beta s during the treatment of myopia, which could enhance the refractive ability and axial length of the eye. Immunohistochemical staining, real-time fluorescent quantitative PCR, and immunoblotting results showed a significant upregulation of MMP2 and NF-kappa B levels, and a significant downregulation of COL-I expression in the TGF-beta treated eyes, suggesting that NF-kappa B and MMP2 are involved in the signaling pathways of TGF-beta s induced myopia and axial elongation. Moreover, the expression levels of IL-6, IL-8, MCP-1, IL-1 beta, TNF-alpha, TAK1, and NF-kappa B in the retina were all significantly elevated. This indicates that TGF-beta stimulates the inflammatory response of retinal pigment epithelial cells through the TAK1-NF-kappa B signaling pathway. In conclusion, this study suggests that TGF-beta promotes the progression of myopia by enhancing intraocular inflammation.
引用
收藏
页码：34 / 42
页数：9
相关论文
共 57 条
[1]   Circulating Vitreous microRNA as Possible Biomarker in High Myopic Eyes with Macular Hole [J].
Ando, Yoshimasa ;
Keino, Hiroshi ;
Inoue, Makoto ;
Hirota, Kazunari ;
Takahashi, Hiroyuki ;
Sano, Kimihiko ;
Koto, Takashi ;
Sato, Tomohito ;
Takeuchi, Masaru ;
Hirakata, Akito .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (07)
[2]   Transient activation of NF-κB through a TAK1/IKK kinase pathway by TGF-β1 inhibits AP-1/SMAD signaling and apoptosis:: implications in liver tumor formation [J].
Arsura, M ;
Panta, GR ;
Bilyeu, JD ;
Cavin, LG ;
Sovak, MA ;
Oliver, AA ;
Factor, V ;
Heuchel, R ;
MErcurio, F ;
Thorgeirsson, SS ;
Sonenshein, GE .
ONCOGENE, 2003, 22 (03) :412-425
[3]  
Asano Kazuhito, 2010, Int J Chron Obstruct Pulmon Dis, V5, P277
[4]   Phosphatidylinositol 3-kinase function is required for transforming growth factor β-mediated epithelial to mesenchymal transition and cell migration [J].
Bakin, AV ;
Tomlinson, AK ;
Bhowmick, NA ;
Moses, HL ;
Arteaga, CL .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (47) :36803-36810
[5]  
Bitzer M, 2000, GENE DEV, V14, P187
[6]   The two NF-κB activation pathways and their role in innate and adaptive immunity [J].
Bonizzi, G ;
Karin, M .
TRENDS IN IMMUNOLOGY, 2004, 25 (06) :280-288
[7]   Candidate pathways for retina to scleral signaling in refractive eye growth [J].
Brown, Dillon M. ;
Mazade, Reece ;
Clarkson-Townsend, Danielle ;
Hogan, Kelleigh ;
Roy, Pooja M. Datta ;
Pardue, Machelle T. .
EXPERIMENTAL EYE RESEARCH, 2022, 219
[8]   The tree shrews: adjuncts and alternatives to primates as models for biomedical research [J].
Cao, J ;
Yang, EB ;
Su, JJ ;
Li, Y ;
Chow, P .
JOURNAL OF MEDICAL PRIMATOLOGY, 2003, 32 (03) :123-130
[9]   Altered TGF-β2 and bFGF expression in scleral desmocytes from an experimentally-induced myopia guinea pig model [J].
Chen, Bo-Yu ;
Wang, Chao-Ying ;
Chen, Wei-Yi ;
Ma, Jing-Xue .
GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 2013, 251 (04) :1133-1144
[10]   Effect of SMAD7 gene overexpression on TGF-β1-induced profibrotic responses in fibroblasts derived from Peyronie's plaque [J].
Choi, Min Ji ;
Song, Kang-Moon ;
Park, Jin-Mi ;
Kwon, Mi-Hye ;
Kwon, Ki-Dong ;
Park, Soo-Hwan ;
Ryu, Dong-Soo ;
Ryu, Ji-Kan ;
Suh, Jun-Kyu .
ASIAN JOURNAL OF ANDROLOGY, 2015, 17 (03) :487-492
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