Structural characterization and anti-inflammatory activity of polysaccharides from Astragalus membranaceus

In this study, two homogeneous polysaccharides (APS-A1 and APS-B1) were isolated from Astragalus mem-branaceus by DEAE-52 cellulose and Sephadex G-100 column chromatography. Their chemical structures were characterized by molecular weight distribution, monosaccharide composition, infrared spectrum, methylation analysis, and NMR. The results revealed that APS-A1 (2.62 x 106 Da) was a 1,4-alpha-D-Glcp backbone with a 1,4,6-alpha-D-Glcp branch every ten residues. APS-B1 (4.95 x 106 Da) was a heteropolysaccharide composed of glucose, galactose, and arabinose (75.24:17.27:19.35). Its backbone consisted of 1,4-alpha-D-Glcp, 1,4,6-alpha-D-Glcp, 1,5-alpha-L-Araf and the sidechains composed of 1,6-alpha-D-Galp and T-alpha/beta-Glcp. Bioactivity assays showed that APS-A1 and APS-B1 had potential anti-inflammatory activity. They could inhibit the production of inflammatory factors (TNF-alpha, IL-6, and MCP-1) in LPS-stimulated RAW264.7 macrophages via NF-kappa B and MAPK (ERK, JNK) pathways. These results suggested that the two polysaccharides could be potential anti-inflammatory supplements.