Performance of Polydioxanone-Based Membrane in Association with 3D-Printed Bioceramic Scaffolds in Bone Regeneration

被引:9
作者
Pitol-Palin, Leticia [1 ]
Frigerio, Paula Buzo [1 ]
Moura, Juliana [1 ]
Pilatti, Livia [2 ]
Jordao de Oliveira, Leticia Marques [2 ]
Matsubara, Elaine Yoshiko [2 ]
Tunchel, Samy [2 ]
Shibli, Jamil Awad [2 ,3 ]
Blay, Alberto [2 ]
Saska, Sybele [2 ]
Okamoto, Roberta [4 ]
机构
[1] Sao Paulo State Univ UNESP, Aracatuba Dent Sch, Dept Diag & Surg, BR-16015050 Aracatuba, Brazil
[2] M3 Hlth Ind & Comercio Prod Med Odontol & Correla, BR-13212213 Jundiai, Brazil
[3] Univ Guarulhos, Dept Periodontol, Dent Res Div, BR-07009043 Guarulhos, Brazil
[4] Sao Paulo State Univ UNESP, Aracatuba Dent Sch, Dept Basic Sci, BR-16066840 Aracatuba, Brazil
关键词
polydioxanone; 3D-printed scaffolds; beta-tricalcium phosphate; hydroxyapatite; bone regeneration; guided tissue regeneration; additive manufacture; synthetic polymer; CALCIUM-PHOSPHATE; GRAFT MATERIALS; RECONSTRUCTION; CHEMISTRY; CELLS;
D O I
10.3390/polym15010031
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
This study evaluated the bioactivity of 3D-printed beta-tricalcium phosphate (beta-TCP) scaffolds or hydroxyapatite (HA) scaffolds associated with polydioxanone (PDO) membrane (Plenum((R)) Guide) for guided bone regeneration in rats. Fifty-four rats were divided into three groups (n = 18 animals): autogenous bone + PDO membrane (Auto/PG); 3D-printed beta-TCP + PDO membrane (TCP/PG); and 3D-printed HA + PDO membrane (HA/PG). A surgical defect in the parietal bone was made and filled with the respective scaffolds and PDO membrane. The animals were euthanized 7, 30, and 60 days after the surgical procedure for micro-CT, histomorphometric, and immunolabeling analyses. Micro-CT showed an increase in trabecular thickness and a decrease in trabecular separation, even with similar bone volume percentages between TCP/PG and HA/PG vs. Auto/PG. Histometric analysis showed increased bone formation at 30 days in the groups compared to 7 days postoperatively. Immunolabeling analysis showed an increase in proteins related to bone formation at 30 days, and both groups showed a similar immunolabeling pattern. This study concludes that 3D-printed scaffolds associated with PDO membrane (Plenum(R) Guide) present similar results to autogenous bone for bone regeneration.
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页数:18
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