Development and comparison of parallel reaction monitoring and data-independent acquisition methods for quantitative analysis of hydrophilic compounds in white tea

被引：2

作者：

Wang, Dan ^{[1
]}

Yu, Zhihao ^{[1
]}

Guo, Jie ^{[1
]}

Liu, Meiyan ^{[1
]}

Guan, Mengdi ^{[1
]}

Gu, Ying ^{[1
]}

Li, Siyu ^{[1
]}

Ren, Dabing ^{[1
]}

Yi, Lunzhao ^{[1
]}

机构：

[1] Kunming Univ Sci & Technol, Fac Food Sci & Engn, Kunming 650500, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2024年 / 1715卷

关键词：

Parallel reaction monitoring; Data-independent acquisition; Quantitative analysis; Hydrophilic compounds; White tea; INTERACTION LIQUID-CHROMATOGRAPHY; FREE AMINO-ACIDS; RESOLUTION MASS-SPECTROMETRY; RAPID-DETERMINATION; QUANTIFICATION; TASTE; NUCLEOSIDES; IDENTIFICATION; NUCLEOTIDES; NUCLEOBASES;

D O I：

10.1016/j.chroma.2023.464601

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present work, parallel reaction monitoring (PRM) and data-independent acquisition (DIA) methods were developed for the accurate quantitation of amino acids, alkaloids nucleosides and nucleotides in tea. The quality peaks were significantly enhanced by optimizing the LC elution procedure, HCD voltage, MS resolution, and scanning event. Both methods were validated with good liner linearity (0.004-200 mu g/mL), LODs (0.001-0.309 mu g/mL for PRM and 0.001-0.564 mu g/mL for DIA). Applied to white tea sample, the contents of these hydrophilic compounds were range from 34,655.39 to 70,586.14 mg/kg, and caffeine (32,529.02 mg/kg) and theanine (5483.46 mg/kg) were determined as the most abundant ones. Based on the quantitation data set, the white tea samples from Puer, Lincang and Xishuangbanna were clearly discriminated using multivariate data analysis. The results of the present works show that PRM and DIA have great potential in quantitative analysis of multiple hydrophilic compounds in food samples.

引用

页数：13