Hyaluronic acid-engineered Bcl-2 inhibitor nanocrystals for site-specific delivery to breast tumor cells

被引：3
|
作者：
Panwar, Dilip [1 ]
Thakor, Pradip [1 ]
Sharma, Madhu [2 ]
Bakshi, Avijit Kumar [2 ]
Bhavana, Valamla [1 ]
Srivastava, Vaibhavi [1 ]
Mishra, Prabhat Ranjan [2 ]
Singh, Shashi Bala [3 ]
Mehra, Neelesh Kumar [1 ]
机构：
[1] Natl Inst Pharmaceut Educ & Res NIPER, Dept Pharmaceut, Pharmaceut Nanotechnol Res Lab, Hyderabad 500037, Telangana, India
[2] Council Sci & Ind Res Cent Drug Res Inst CSIR CDRI, Div Pharmaceut, Lucknow 226017, Uttar Pradesh, India
[3] Natl Inst Pharmaceut Educ & Res NIPER, Dept Biol Sci, Hyderabad 500037, Telangana, India
来源：
NANOMEDICINE | 2023年 / 18卷 / 15期
关键词：
breast cancer; glycosaminoglycan; hyaluronic acid; nanocrystals; repurposing; venetoclax; DRUG-DELIVERY; NANOPARTICLES; DOXORUBICIN; PACLITAXEL; PH;
D O I：
10.2217/nnm-2023-0132
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aim: This investigation aims to repurpose venetoclax using hyaluronic acid-coated venetoclax nanocrystals (HA-VEN-NCs) to target breast cancer. Materials & methods: An antisolvent precipitation method was used to fabricate the nanocrystals and optimize them using central composite design. Hyaluronic acid (HA)-coated and -uncoated nanocrystals were compared in terms of in vitro drug release, cell line studies, CD44-expressing breast tumor cell binding capability and anticancer activity. Results: HA-VEN-NCs and venetoclax nanocrystals (VEN-NCs) showed pH-responsive drug-release behavior, exhibiting sustained release at pH 6.8. Our extensive in vitro cell line investigation showed that HA-VEN-NCs efficiently bind to CD44-expressing breast tumor cells and possess excellent anticancer activity (half maximal inhibitory concentration: 2.00 & mu;g/ml) compared with VEN-NCs. Conclusion: Our findings anticipate that HA-VEN-NCs could serve as valuable nanoplatforms for cancer treatments in the future.
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收藏
页码：1005 / 1023
页数：19
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