Evaluation of an in-house IgM/IgG lateral flow assay for serodiagnosis of human brucellosis

被引：1

作者：

Shome, Rajeswari ^{[1
,3
]}

Patra, Sudipta ^{[1
]}

Sahib, Muneera Mohamed ^{[1
]}

Shanmugam, G. ^{[1
]}

Dubey, Shiva ^{[1
]}

Skariah, Somy ^{[1
]}

Shamshad, Samer ^{[1
]}

Barman, Nagendra Nath ^{[2
]}

Bora, Durlav Prasad ^{[2
]}

Shome, Arijit ^{[2
]}

Mohandoss, Nagalingam ^{[1
]}

Shome, Bibek Ranjan ^{[1
]}

机构：

[1] Indian Council Agr Res, Natl Inst Vet Epidemiol & Dis Informat, Bengalore 560064, India

[2] Assam Agr Univ, Coll Vet Sci, Gauhati 781022, India

[3] Management & Sci Univ, Int Med Sch, Dept Microbiol, Bangalore Campus, Bangalore 560054, India

来源：

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY | 2023年 / 42卷

关键词：

ELISA; Human brucellosis; Lateral flow assay (LFA); RBPT; SAT; ROSE-BENGAL; DIAGNOSIS; SPECIFICITY; SENSITIVITY; CATTLE; AREAS;

D O I：

10.1016/j.ijmmb.2023.01.006

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

This study aimed to evaluate the diagnostic efficacy of an in-house lateral flow assay (LFA) for the detection of IgM/IgG anti-Brucella antibodies for rapid serodiagnosis of human brucellosis. Three groups of sera samples including 476 from high-risk individuals, 27 from culture-confirmed patients, and 43 from healthy blood donors were used for evaluation of LFA. In comparison with iELISA, the sensitivity, specificity, and accuracy of LFA were >95%, >99%, and 99% respectively. Considering the very good agreement, accuracy, simplicity, and rapidity, LFAs might be useful as a point of care test for the diagnosis of human brucellosis in resource-limited laboratories.

引用

页码：55 / 58

页数：4

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