Evaluation of an in-house IgM/IgG lateral flow assay for serodiagnosis of human brucellosis

被引：1
|
作者：
Shome, Rajeswari [1 ,3 ]
Patra, Sudipta [1 ]
Sahib, Muneera Mohamed [1 ]
Shanmugam, G. [1 ]
Dubey, Shiva [1 ]
Skariah, Somy [1 ]
Shamshad, Samer [1 ]
Barman, Nagendra Nath [2 ]
Bora, Durlav Prasad [2 ]
Shome, Arijit [2 ]
Mohandoss, Nagalingam [1 ]
Shome, Bibek Ranjan [1 ]
机构：
[1] Indian Council Agr Res, Natl Inst Vet Epidemiol & Dis Informat, Bengalore 560064, India
[2] Assam Agr Univ, Coll Vet Sci, Gauhati 781022, India
[3] Management & Sci Univ, Int Med Sch, Dept Microbiol, Bangalore Campus, Bangalore 560054, India
来源：
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY | 2023年 / 42卷
关键词：
ELISA; Human brucellosis; Lateral flow assay (LFA); RBPT; SAT; ROSE-BENGAL; DIAGNOSIS; SPECIFICITY; SENSITIVITY; CATTLE; AREAS;
D O I：
10.1016/j.ijmmb.2023.01.006
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
This study aimed to evaluate the diagnostic efficacy of an in-house lateral flow assay (LFA) for the detection of IgM/IgG anti-Brucella antibodies for rapid serodiagnosis of human brucellosis. Three groups of sera samples including 476 from high-risk individuals, 27 from culture-confirmed patients, and 43 from healthy blood donors were used for evaluation of LFA. In comparison with iELISA, the sensitivity, specificity, and accuracy of LFA were >95%, >99%, and 99% respectively. Considering the very good agreement, accuracy, simplicity, and rapidity, LFAs might be useful as a point of care test for the diagnosis of human brucellosis in resource-limited laboratories.
引用
收藏
页码：55 / 58
页数：4
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