Molecular Cloning and Functional Characterization of Oxidosqualene Cyclases from Panax vietnamensis

被引：2

作者：

Wang, Yibo ^{[1
,2
,3
]}

Wang, Baojie ^{[2
,3
]}

Xu, Furong ^{[2
,3
]}

Ma, Xiaohui ^{[1
,2
,3
]}

机构：

[1] Yunnan Univ Chinese Med, Yunnan Prov Dept Educ Subst Benchmark Res Ethn Med, Key Lab, Kunming 650500, Peoples R China

[2] Yunnan Univ Chinese Med, Coll Chinese Mat Med, Kunming 650500, Peoples R China

[3] Yunnan Univ Chinese Med, Yunnan Key Lab Southern Med Utilizat, Kunming 650500, Peoples R China

来源：

CHEMISTRY & BIODIVERSITY | 2023年 / 20卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Panax vietnamensis; oxidosqualene cyclase; beta-amyrin; cycloartenol; dammarenediol-II; BETA-AMYRIN SYNTHASE; BIOSYNTHESIS; TRITERPENE; GENE;

D O I：

10.1002/cbdv.202200874

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Panax vietnamensis is a valuable medicinal resource with promising preclinical applications. Ginsenosides, which are triterpenoids, are the primary active components in P. vietnamensis. Oxidosqualene cyclases (OSCs) catalyze the formation of the basic skeleton of triterpenes from 2,3-oxidosqualene, which is a crucial step in the biosynthesis of triterpenoids. The OSCs involved in triterpenoid biosynthesis in P. vietnamensis have not yet been characterized. Four OSC genes (PvOSC1-4) were cloned from P. vietnamensis and functionally characterized via heterologous expression in yeast. Transgenic yeast expressing PvOSC1, PvOSC3, and PvOSC4 produced the corresponding products beta-amyrin, cycloartenol, and dammarenediol-II, respectively. PvOSC1, PvOSC3, and PvOSC4 are monofunctional OSCs. In this study, we characterized three PvOSC genes, providing a better understanding of the biosynthesis of triterpenoids in P. vietnamensis and the multiple choices of plant OSCs for metabolic engineering in yeast and other hosts.

引用

页数：10

共 50 条

[31] Cloning of 2,3-oxidosqualene Cyclase Gene from Panax quinquefolius and Its Expression Pattern
Qiong WU
Yixuan HAN
Jianqing ZHANG
Medicinal Plant, 2013, (Z1) : 8 - 12
[32] Cloning and Characterization of Guanylyl Cyclases from the European Green Crab
Gunderson, J. L.
Maclea, K. S.
Covi, J. A.
Chang, S. A.
Chang, E. S.
Mykles, D. L.
INTEGRATIVE AND COMPARATIVE BIOLOGY, 2010, 50 : E236 - E236
[33] Molecular cloning and functional analysis of squalene synthase (SS) in Panax notoginseng
Jiang, Dan
Rong, Qixian
Chen, Yijun
Yuan, Qingjun
Shen, Ye
Guo, Juan
Yang, Yirui
Zha, Liangping
Wu, Huixiao
Huang, Luqi
Liu, Chunsheng
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2017, 95 : 658 - 666
[34] Molecular cloning, expression, and functional characterization of a novel member of the CD38 family of ADP-ribosyl cyclases
Adebanjo, OA
Koval, A
Moonga, BS
Wu, XB
Yao, S
Bevis, PJR
Kumegawa, M
Zaidi, I
Sun, L
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 273 (03) : 884 - 889
[35] Two branches of the lupeol synthase gene in the molecular evolution of plant oxidosqualene cyclases
Shibuya, M
Zhang, H
Endo, A
Shishikura, K
Kushiro, T
Ebizuka, Y
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 266 (01): : 302 - 307
[36] Molecular cloning and characterization of the gene encoding squalene epoxidase in Panax notoginseng
He, Fengmei
Zhu, Yongping
He, Mingxiong
Zhang, Yizheng
DNA SEQUENCE, 2008, 19 (03): : 270 - 273
[37] Molecular cloning and functional characterization of MnSOD from Dunaliella salina
Zhang, Shu
Li, Xin Ran
Xu, Hui
Cao, Yu
Ma, Shu Han
Cao, Yi
Qiao, Dairong
JOURNAL OF BASIC MICROBIOLOGY, 2014, 54 (05) : 438 - 447
[38] Cloning and characterization of a 2,3-oxidosqualene cyclase from Eleutherococcus trifoliatus
Ma, Li-Ting
Wang, Sheng-Yang
Tseng, Yen-Hsueh
Lee, Yi-Ru
Chu, Fang-Hua
HOLZFORSCHUNG, 2013, 67 (04) : 463 - 471
[39] Molecular cloning and expression of SS gene from Panax japonicus
Zhang, Lai
Huang, Yuanshe
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2016, 12 (02) : 524 - 524
[40] Molecular Cloning and Expression of SS Gene from Panax japonicus
Zhang, Lai
Sun, Min
JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2014, 8 : 565 - 569

← 1 2 3 4 5 →