Characterizing CD4 T cell differentiation in mouse small intestine using T cell transfer, lamina propria preparation, and flow cytometry

被引：0
|
作者：
Duan, Jinzhi [1 ]
Sun, Yanan [2 ]
Matute, Juan D. [1 ,3 ]
Blumberg, Richard S. [1 ]
机构：
[1] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Gastroenterol, Boston, MA 02115 USA
[2] Boston Univ, Sch Med, Boston Med Ctr, Dept Med,Sect Hematol Med Oncol, Boston, MA 02118 USA
[3] Harvard Med Sch, Massachusetts Gen Hosp, Dept Pediat, Div Neonatol & Newborn Med, Boston, MA 02114 USA
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 03期
关键词：
Flow Cytometry/Mass Cytometry; Immunology;
D O I：
10.1016/j.xpro.2023.102485
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Studying gene function in T cells is crucial for understanding physiology and dis-ease pathogenesis. Here, we provide a protocol to examine the role of specific genes in CD4+ T cell differentiation in the intestine. We describe steps for isolating naive CD4+ T cells from mouse spleens and transferring them to recipient mice. We detail procedures to isolate lamina propria cells and analyze CD4+ T subsets using flow cytometry. This protocol is useful in the study of mucosal immune functions. For complete details on the use and execution of this protocol, please refer to Duan et al.1
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页数：18
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