RPN1 promotes the proliferation and invasion of breast cancer cells by activating the PI3K/AKT/mTOR signaling pathway

被引:6
作者
Shen, Wei-juan [1 ,2 ]
Zhang, Yi [1 ,2 ]
机构
[1] Jiangsu Univ, Changzhou Wujin Peoples Hosp, Dept Hepatobiliary Surg, Dept Breast Surg,Wujin Hosp, Changzhou 213004, Jiangsu, Peoples R China
[2] Xuzhou Med Univ, Dept Breast Surg, Wujin Clin Coll, Changzhou 213004, Jiangsu, Peoples R China
关键词
RPN1; Breast cancer; PI3K/AKT/mTOR; Migration; Invasion; MIGRATION; SURVIVAL; GROWTH;
D O I
10.1007/s12672-024-00875-8
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Ribophorin I (RPN1), a part of an N-oligosaccharyl-transferase complex, plays a vital role in the development of multiple cancers. However, its biological role in breast cancer has not been completely clarified. The RPN1 expression level was measured in breast cancer tissues and breast cancer cell lines (MCF7) using RT-qPCR. After down-regulating RPN1 expression by shRNA, the effects of RPN1 on the proliferation, migration and invasion of MCF7 cells were examined. Mechanistically, we assessed the effect of RPN1 on the PI3K/ AKT/mTOR signaling pathway. We found that RPN1 level was up-regulated in breast cancer tissues and cells compared with adjacent non-tumor tissues or MCF10A cells. RPN1 knockdown induced apoptosis and attenuated the proliferation, migration, and invasion of MCF7 cells. Moreover, RPN1 knockdown lowered the levels of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR, which were rescued by 740Y-P, a PI3K activator. 740Y-P also reversed the effects of RPN1 knockdown on apoptosis, proliferation, migration, and invasion in MCF7 cells. Taken together, RPN1 promotes the proliferation, migration, and invasion of breast cancer cells via the PI3K/AKT/mTOR signaling pathway.
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页数:9
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