Spliceostatin C, a component of a microbial bioherbicide, is a potent phytotoxin that inhibits the spliceosome

被引：9

作者：

Bajsa-Hirschel, Joanna ^{[1
]}

Pan, Zhiqiang ^{[1
]}

Pandey, Pankaj ^{[2
]}

Asolkar, Ratnakar N. ^{[3
]}

Chittiboyina, Amar G. ^{[2
]}

Boddy, Louis ^{[3
]}

Machingura, Marylou C. ^{[4
]}

Duke, Stephen O. ^{[2
]}

机构：

[1] Agr Res Serv, USDA, Nat Prod Utilizat Res Unit, University, MS USA

[2] Univ Mississippi, Natl Ctr Nat Prod Res, Sch Pharm, University, MS USA

[3] Bioceres Crop Solut, Davis, CA USA

[4] Georgia Southern Univ, Dept Biol, Savannah, GA USA

来源：

FRONTIERS IN PLANT SCIENCE | 2023年 / 13卷

关键词：

splicing; splicing inhibitors; spliceostatin; bioherbicide; arabidopsis; proteomics; GUIDED DISCOVERY; STOMATAL CLOSURE; NATURAL-PRODUCTS; BETA-SUBUNIT; G-PROTEIN; RESPONSES; ARABIDOPSIS; HERBOXIDIENE; INTERACTS;

D O I：

10.3389/fpls.2022.1019938

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Spliceostatin C (SPC) is a component of a bioherbicide isolated from the soil bacterium Burkholderia rinojensis. The chemical structure of SPC closely resembles spliceostatin A (SPA) which was characterized as an anticancer agent and splicing inhibitor. SPC inhibited the growth of Arabidopsis thaliana seedlings with an IC50 value of 2.2 mu M. The seedlings exposed to SPC displayed a significant response with decreased root length and number and inhibition of gravitropism. Reverse transcriptase semi-quantitative PCR (RT-sqPCR) analyses of 19 selected genes demonstrated the active impact of SPC on the quality and quantity of transcripts that underwent intron rearrangements as well as up or down expression upon exposure to SPC. Qualitative and quantitative proteomic profiles identified 66 proteins that were significantly affected by SPC treatment. Further proteomics data analysis revealed that spliceostatin C induces hormone-related responses in Arabidopsis seedlings. In silico binding studies showed that SPC binds to a pocket between the SF3B3 and PF5A of the spliceosome.

引用

页数：17

共 64 条

[1] Family-wide evaluation of RAPID ALKALINIZATION FACTOR peptides [J].

Abarca, Alicia ;

Franck, Christina M. ;

Zipfel, Cyril .

PLANT PHYSIOLOGY, 2021, 187 (02) :996-1010

[2] Hormone interactions in stomatal function [J].

Acharya, Biswa R. ;

Assmann, Sarah M. .

PLANT MOLECULAR BIOLOGY, 2009, 69 (04) :451-462

[3] Herboxidiene triggers splicing repression and abiotic stress responses in plants [J].

AlShareef, Sahar ;

Ling, Yu ;

Butt, Haroon ;

Mariappan, Kiruthiga G. ;

Benhamed, Moussa ;

Mahfouz, Magdy M. .

BMC GENOMICS, 2017, 18

[4] Potent induction of Arabidopsis thaliana flowering by elevated growth temperature [J].

Balasubramanian, Sureshkumar ;

Sureshkumar, Sridevi ;

Lempe, Janne ;

Weigel, Detlef .

PLOS GENETICS, 2006, 2 (07) :980-989

[5] ABA-induced NO generation and stomatal closure in Arabidopsis are dependent on H2O2 synthesis [J].

Bright, J ;

Desikan, R ;

Hancock, JT ;

Weir, IS ;

Neill, SJ .

PLANT JOURNAL, 2006, 45 (01) :113-122

[6] MIQE precis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments [J].

Bustin, Stephen A. ;

Beaulieu, Jean-Francois ;

Huggett, Jim ;

Jaggi, Rolf ;

Kibenge, Frederick S. B. ;

Olsvik, Pal A. ;

Penning, Louis C. ;

Toegel, Stefan .

BMC MOLECULAR BIOLOGY, 2010, 11

[7] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[8] A Comprehensive Analysis of RALF Proteins in Green Plants Suggests There Are Two Distinct Functional Groups [J].

Campbell, Liam ;

Turner, Simon R. .

FRONTIERS IN PLANT SCIENCE, 2017, 8

[9] Alternative Splicing and Protein Diversity: Plants Versus Animals [J].

Chaudhary, Saurabh ;

Khokhar, Waqas ;

Jabre, Ibtissam ;

Reddy, Anireddy S. N. ;

Byrne, Lee J. ;

Wilson, Cornelia M. ;

Syed, Naeem H. .

FRONTIERS IN PLANT SCIENCE, 2019, 10

[10] The Cytosolic/Nuclear HSC70 and HSP90 Molecular Chaperones Are Important for Stomatal Closure and Modulate Abscisic Acid-Dependent Physiological Responses in Arabidopsis [J].

Clement, Mathilde ;

Leonhardt, Nathalie ;

Droillard, Marie-Jo ;

Reiter, Ilja ;

Montillet, Jean-Luc ;

Genty, Bernard ;

Lauriere, Christiane ;

Nussaume, Laurent ;

Noel, Laurent D. .

PLANT PHYSIOLOGY, 2011, 156 (03) :1481-1492

← 1 2 3 4 5 6 7 →