Establishment and characterization of a new intrahepatic cholangiocarcinoma cell line, ICC-X3

被引：2

作者：

Xu, Hao ^{[1
]}

Luo, Wei ^{[1
]}

Zhao, Zhenjie ^{[1
]}

Miao, Xin ^{[2
]}

Chai, Changpeng ^{[1
]}

Hu, Jinjing ^{[1
]}

Tang, Huan ^{[3
]}

Zhang, Hui ^{[4
]}

Zhou, Wence ^{[3
,4
]}

机构：

[1] Lanzhou Univ, Hosp 1, Forth Dept Gen Surg, 1 Donggang West Rd, Lanzhou 730000, Gansu, Peoples R China

[2] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Anim Virol Minist Agr, Lanzhou 730000, Peoples R China

[3] Lanzhou Univ, Clin Med Coll 2, Lanzhou 730000, Peoples R China

[4] Lanzhou Univ, Hosp 2, Dept Gen Surg, Lanzhou 730000, Peoples R China

来源：

HUMAN CELL | 2023年 / 36卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Intrahepatic cholangiocarcinoma; Cell line; Establishment; Xenograft; Drug resistance; PRIMARY LIVER-CANCER; RISING INCIDENCE; ASIAN PATIENTS; UNITED-STATES; RESISTANCE; EPIDEMIOLOGY; ACCUMULATION; MUTATIONS; CARCINOMA; APOPTOSIS;

D O I：

10.1007/s13577-023-00858-x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Intrahepatic cholangiocarcinoma (ICC) is an aggressive cancer of the biliary tract that is prone to recurrence and metastasis and is characterized by poor sensitivity to chemotherapy and overall prognosis. To address this challenge, the establishment of suitable preclinical models is critical. In this study, we successfully established a new ICC cell line, named ICC-X3, from the satellite lesions of one ICC patient. The cell line was characterized with respect to phenotypic, molecular, biomarker, functional and histological properties. STR confirmed that ICC-X3 was highly consistent with primary tumor tissue. ICC-X3 cells positively expressed CK7, CK19, E-cadherin, vimentin, and Ki67. ICC-X3 was all resistant to gemcitabine, paclitaxel, 5-FU, and oxaliplatin. The cell line was able to rapidly form xenograft tumors which were highly similar to the primary tumor. The missense mutation of TP53 exon was detected in ICC-X3 cells. ICC-X3 can be used as a good experimental model to study the progression, metastasis, and drug resistance of ICC.

引用

页码：854 / 865

页数：12

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