In the mouse cortex, oligodendrocytes regain a plastic capacity, transforming into astrocytes after acute injury

被引：16

作者：

Bai, Xianshu ^{[1
]}

Zhao, Na ^{[1
,10
]}

Koupourtidou, Christina ^{[2
,3
]}

Fang, Li-Pao ^{[1
]}

Schwarz, Veronika ^{[2
,3
]}

Caudal, Laura C. ^{[1
]}

Zhao, Renping ^{[1
,11
]}

Hirrlinger, Johannes ^{[4
,5
]}

Walz, Wolfgang ^{[1
,6
]}

Bian, Shan ^{[7
,8
]}

Huang, Wenhui ^{[1
]}

Ninkovic, Jovica ^{[2
,3
]}

Kirchhoff, Frank ^{[1
,9
]}

Scheller, Anja ^{[1
]}

机构：

[1] Univ Saarland, Ctr Integrat Physiol & Mol Med, Mol Physiol, D-66421 Homburg, Germany

[2] Ludwig Maximilians Univ Munchen, Dept Cell Biol & Anat, D-82152 Martinsried, Germany

[3] Helmholtz Zentrum Munich, Inst Stem Cell Res, D-85764 Munich, Germany

[4] Univ Leipzig, Carl Ludwig Inst Physiol, D-04103 Leipzig, Germany

[5] Max Planck Inst Multidisciplinary Sci, Dept Neurogenet, D-37075 Gottingen, Germany

[6] Univ Saskatchewan, Dept Psychiat, 107 Wiggins Rd, Saskatoon, SK S7N 5E5, Canada

[7] Tongji Univ, Shanghai East Hosp, Inst Regenerat Med, Sch Life Sci & Technol, Shanghai 200092, Peoples R China

[8] Tongji Univ, Frontier Sci Ctr Stem Cell Res, Shanghai 200092, Peoples R China

[9] Univ Med & Pharm Craiova, Expt Res Ctr Normal & Pathol Aging, Craiova 200349, Romania

[10] Univ Saarland, Inst Anat & Cell Biol, D-66421 Homburg, Germany

[11] Univ Saarland, Ctr Integrat Physiol & Mol Med, Biophys, D-66421 Homburg, Germany

来源：

DEVELOPMENTAL CELL | 2023年 / 58卷 / 13期

基金：

欧盟地平线“2020”;

关键词：

FIBRILLARY ACIDIC PROTEIN; CELL FATE DETERMINATION; CENTRAL-NERVOUS-SYSTEM; NG2; GLIA; PROGENITOR CELLS; NMDA RECEPTORS; WHITE-MATTER; STEM-CELLS; DIFFERENTIATION; LINEAGE;

D O I：

10.1016/j.devcel.2023.04.016

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Acute brain injuries evoke various response cascades directing the formation of the glial scar. Here, we report that acute lesions associated with hemorrhagic injuries trigger a re-programming of oligodendrocytes. Single-cell RNA sequencing highlighted a subpopulation of oligodendrocytes activating astroglial genes after acute brain injuries. By using PLP-DsRed1/GFAP-EGFP and PLP-EGFPmem/GFAP-mRFP1 transgenic mice, we visualized this population of oligodendrocytes that we termed AO cells based on their concomitant activity of astro-and oligodendroglial genes. By fate mapping using PLP-and GFAP-split Cre complementation and repeated chronic in vivo imaging with two-photon laser-scanning microscopy, we observed the conversion of oligodendrocytes into astrocytes via the AO cell stage. Such conversion was promoted by local injection of IL-6 and was diminished by IL-6 receptor-neutralizing antibody as well as by inhibiting microglial activation with minocycline. In summary, our findings highlight the plastic potential of oligodendrocytes in acute brain trauma due to microglia-derived IL-6.

引用

页码：1153 / +

页数：23

共 88 条

[1] HETEROTYPIC AND HOMOTYPIC CELLULAR INTERACTIONS INFLUENCING THE GROWTH AND DIFFERENTIATION OF BIPOTENTIAL OLIGODENDROCYTE-TYPE-2 ASTROCYTE PROGENITORS IN CULTURE [J].

AGRESTI, C ;

ALOISI, F ;

LEVI, G .

DEVELOPMENTAL BIOLOGY, 1991, 144 (01) :16-29

[2] NG2 proteoglycan-expressing cells of the adult rat brain: Possible involvement in the formation of glial scar astrocytes following stab wound [J].

Alonso, G .

GLIA, 2005, 49 (03) :318-338

[3]

Ankerhold R, 1999, J NEUROBIOL, V41, P572, DOI 10.1002/(SICI)1097-4695(199912)41:4<572::AID-NEU12>3.3.CO

[4]

2-#

[5] Live imaging of astrocyte responses to acute injury reveals selective juxtavascular proliferation [J].

Bardehle, Sophia ;

Krueger, Martin ;

Buggenthin, Felix ;

Schwausch, Julia ;

Ninkovic, Jovica ;

Clevers, Hans ;

Snippert, Hugo J. ;

Theis, Fabian J. ;

Meyer-Luehmann, Melanie ;

Bechmann, Ingo ;

Dimou, Leda ;

Goetz, Magdalena .

NATURE NEUROSCIENCE, 2013, 16 (05) :580-+

[6] Cytokine Control of Adult Neural Stem Cells Chronic versus Acute Exposure [J].

Bauer, Sylvian .

NEUROIMMUNOMODULATION: FROM FUNDAMENTAL BIOLOGY TO THERAPY, 2009, 1153 :48-56

[7]

BESNARD F, 1991, J BIOL CHEM, V266, P18877

[8] LIF and BMP signaling generate separate and discrete types of GFAP-expressing cells [J].

Bonaguidi, MA ;

McGuire, T ;

Hu, M ;

Kan, LX ;

Samanta, J ;

Kessler, JA .

DEVELOPMENT, 2005, 132 (24) :5503-5514

[9] GFAP PROMOTER DIRECTS ASTROCYTE-SPECIFIC EXPRESSION IN TRANSGENIC MICE [J].

BRENNER, M ;

KISSEBERTH, WC ;

SU, Y ;

BESNARD, F ;

MESSING, A .

JOURNAL OF NEUROSCIENCE, 1994, 14 (03) :1030-1037

[10] Origin and progeny of reactive gliosis:: A source of multipotent cells in the injured brain [J].

Buffo, Annalisa ;

Rite, Inmaculada ;

Tripathi, Pratibha ;

Lepier, Alexandra ;

Colak, Dilek ;

Horn, Ana-Paula ;

Mori, Tetsuji ;

Goetz, Magdalena .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (09) :3581-3586

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