Investigation of Plasma-Derived Lipidome Profiles in Experimental Cerebral Malaria in a Mouse Model Study

被引:3
作者
Batarseh, Amani M. [1 ,2 ]
Vafaee, Fatemeh [3 ,4 ,5 ]
Hosseini-Beheshti, Elham [6 ]
Safarchi, Azadeh [3 ]
Chen, Alex [7 ]
Cohen, Amy [6 ]
Juillard, Annette [6 ]
Hunt, Nicholas Henry [8 ]
Mariani, Michael [9 ]
Mitchell, Todd [10 ,11 ]
Grau, Georges Emile Raymond [6 ]
机构
[1] Univ Sydney, Sydney Knowledge Hub, BCAL Dx Ltd, Merewether Bldg, Sydney, NSW 2006, Australia
[2] BCAL Dx Ltd, Suite 506,50 Clarence St, Sydney, NSW 2000, Australia
[3] Univ New South Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia
[4] Univ New South Wales, UNSW Data Sci Hub, Sydney, NSW 2052, Australia
[5] Omni ai Pty Ltd, Sydney, NSW 2035, Australia
[6] Univ Sydney, Fac Med & Hlth, Sch Med Sci, Vasc Immunol Unit,Discipline Pathol, Sydney, NSW 2000, Australia
[7] Thermo Fisher Sci, Scoresby, Vic 3179, Australia
[8] Univ Sydney, Bosch Inst, Fac Med & Hlth, Sch Med Sci, Sydney, NSW 2000, Australia
[9] Thermo Fisher Sci, N Ryde, NSW 2113, Australia
[10] Univ Wollongong, Fac Sci Med & Hlth, Sch Med, Wollongong, NSW 2522, Australia
[11] Univ Wollongong, Illawarra Hlth & Med Res Inst, Wollongong, NSW 2522, Australia
基金
英国医学研究理事会;
关键词
cerebral malaria; lipidome; mouse model; Plasmodium spp; EXTRACELLULAR VESICLES; FALCIPARUM-MALARIA; EMERGING ROLES; PATHOGENESIS; IMMUNE; MICROPARTICLES; SEQUESTRATION; MICROVESICLES; PLATELETS; FEATURES;
D O I
10.3390/ijms24010501
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cerebral malaria (CM), a fatal complication of Plasmodium infection that affects children, especially under the age of five, in sub-Saharan Africa and adults in South-East Asia, results from incompletely understood pathogenetic mechanisms. Increased release of circulating miRNA, proteins, lipids and extracellular vesicles has been found in CM patients and experimental mouse models. We compared lipid profiles derived from the plasma of CBA mice infected with Plasmodium berghei ANKA (PbA), which causes CM, to those from Plasmodium yoelii (Py), which does not. We previously showed that platelet-free plasma (18k fractions enriched from plasma) contains a high number of extracellular vesicles (EVs). Here, we found that this fraction produced at the time of CM differed dramatically from those of non-CM mice, despite identical levels of parasitaemia. Using high-resolution liquid chromatography-mass spectrometry (LCMS), we identified over 300 lipid species within 12 lipid classes. We identified 45 and 75 lipid species, mostly including glycerolipids and phospholipids, with significantly altered concentrations in PbA-infected mice compared to Py-infected and uninfected mice, respectively. Total lysophosphatidylethanolamine (LPE) levels were significantly lower in PbA infection compared to Py infection and controls. These results suggest that experimental CM could be characterised by specific changes in the lipid composition of the 18k fraction containing circulating EVs and can be considered an appropriate model to study the role of lipids in the pathophysiology of CM.
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页数:16
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