Non-filamentous sludge bulking induced by exopolysaccharide variation in structure and properties during aerobic granulation

被引:14
作者
Wang, Shuai [1 ,2 ]
Wang, Gonglei [1 ]
Yan, Peng [1 ]
Chen, Youpeng [1 ]
Fang, Fang [1 ]
Guo, Jinsong [1 ]
机构
[1] Chongqing Univ, Coll Environm & Ecol, Chongqing 400045, Peoples R China
[2] Peking Univ, Coll Environm Sci & Engn, Beijing 100871, Peoples R China
基金
中国国家自然科学基金;
关键词
Aerobic granulation; Extracellular polymeric substances; Non-filamentous sludge bulking; Microbial community; Feeding strategies; C-DI-GMP; ACTIVATED-SLUDGE; DIFFERENT PH; STABILITY; SELECTION; GRANULES; ANAMMOX; DENITRIFICATION; STRATEGIES; BACTERIA;
D O I
10.1016/j.scitotenv.2023.162786
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The forming mechanism of non-filamentous sludge bulking during aerobic granulation were investigated basing on three feeding strategies (R1 direct aeration after fast feeding, R2 anaerobic stirring after fast feeding and R3 anaerobic plug-flow slow feeding). Results showed that strong selection stress (shortening settling time) led to a sharp flocs washout and the subsequent increase of food to microorganisms (F/M) in R1 and R3 reactors, but not found in R2 due to the different strat-egies of feeding modes. With the increase of F/M, zeta potential and hydrophobicity of sludge surfaces significantly de-creased and thus leading to an enhanced repulsive force and energy barriers for sludge aggregation. Particularly, when F/M exceeded 1.2 kgCOD/(kgMLSS center dot d), non-filamentous sludge bulking was ultimately triggered in R1 and R3. Further analysis showed that massive extracellular exopolysaccharide (PS) accumulated on the surfaces of non-filamentous bulking sludge due to the increased abundance of the microorganisms related to PS secretion during sludge bulking. In addition, significantly increased intracellular second messenger (c-di-GMP), a key substance regulating PS biosynthesis, was confirmed via its concentration determination as well as microbial function prediction analysis, which played a critical role in sludge bulking. Combing with the systematic detection from surface plasmon resonance system, rheometer and size-exclusion chromatography-multiangle laser light detection-refractive index system, higher molecular weight, compact conformation, higher viscosity and higher hydrophilicity was determined in sludge bulking PS relative to PS extracted from non-filamentous bulking sludge. Clearly, the changes of PS (content, structures and properties) driven by c-di-GMP are the dominant mechanism for the formation of non-filamentous sludge bulking during aerobic granulation. This work could provide theoretical support for successful start-up and application of aerobic granular sludge technology.
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页数:11
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