Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication

被引：25
作者：
Chau, Bao-An [1 ,2 ]
Chen, Venessa [1 ,2 ]
Cochrane, Alan W. [3 ]
Parent, Leslie J. [4 ,5 ]
Mouland, Andrew J. [1 ,2 ,6 ]
机构：
[1] Jewish Gen Hosp, HIV RNA Trafficking Lab 1, Lady Davis Inst, Montreal, PQ H3T 1E2, Canada
[2] McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3A 2B4, Canada
[3] Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada
[4] Penn State Coll Med, Dept Med & Microbiol, Div Infect Dis & Epidemiol, Hershey, PA 17033 USA
[5] Penn State Coll Med, Dept Immunol, Div Infect Dis & Epidemiol, Hershey, PA 17033 USA
[6] McGill Univ, Dept Med, Montreal, PQ H4A 3J1, Canada
来源：
CELL REPORTS | 2023年 / 42卷 / 01期
基金：
加拿大健康研究院; 美国国家卫生研究院;
关键词：
RNA-BINDING DOMAIN; CORONAVIRUS; COVID-19; THERAPEUTICS; TRANSITIONS; AGGREGATION; GRANULES; KINASES; LESSONS; ORIGIN;
D O I：
10.1016/j.celrep.2022.111968
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The leap of retroviruses and coronaviruses from animal hosts to humans has led to two ongoing pandemics and tens of millions of deaths worldwide. Retrovirus and coronavirus nucleocapsid proteins have been stud-ied extensively as potential drug targets due to their central roles in virus replication, among which is their capacity to bind their respective genomic RNAs for packaging into nascent virions. This review focuses on fundamental studies of these nucleocapsid proteins and how their intrinsic abilities to condense through liquid-liquid phase separation (LLPS) contribute to viral replication. Therapeutic targeting of these conden-sates and methodological advances are also described to address future questions on how phase separation contributes to viral replication.
引用
收藏
页数：15
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