Nanocrystalline Cellulose as a Versatile Engineering Material for Extrusion-Based Bioprinting

被引:4
作者
Read, Sophia A. [1 ,2 ]
Go, Chee Shuen [1 ,2 ]
Ferreira, Miguel J. S. [1 ,2 ]
Ligorio, Cosimo [2 ,3 ]
Kimber, Susan J. [4 ]
Dumanli, Ahu G. [2 ,3 ]
Domingos, Marco A. N. [1 ,2 ]
机构
[1] Univ Manchester, Fac Sci & Engn, Sch Engn, Dept Mech Aerospace & Civil Engn, Manchester M13 9PL, England
[2] Univ Manchester, Henry Royce Inst, Manchester M13 9PL, England
[3] Univ Manchester, Fac Sci & Engn, Sch Nat Sci, Dept Mat, Manchester M13 9PL, England
[4] Univ Manchester, Sch Biol Sci, Fac Biol Med & Hlth, Div Cell Matrix Biol & Regenerat Med, Manchester M13 9PT, England
基金
英国工程与自然科学研究理事会;
关键词
3D bioprinting; tissue engineering; biomaterials; alginate; cellulose nanocrystals; bioinks; ALGINATE; GROWTH; CELL; CHONDROCYTES; SUSPENSIONS;
D O I
10.3390/pharmaceutics15102432
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Naturally derived polysaccharide-based hydrogels, such as alginate, are frequently used in the design of bioinks for 3D bioprinting. Traditionally, the formulation of such bioinks requires the use of pre-reticulated materials with low viscosities, which favour cell viability but can negatively influence the resolution and shape fidelity of the printed constructs. In this work, we propose the use of cellulose nanocrystals (CNCs) as a rheological modifier to improve the printability of alginate-based bioinks whilst ensuring a high viability of encapsulated cells. Through rheological analysis, we demonstrate that the addition of CNCs (1% and 2% (w/v)) to alginate hydrogels (1% (w/v)) improves shear-thinning behaviour and mechanical stability, resulting in the high-fidelity printing of constructs with superior resolution. Importantly, LIVE/DEAD results confirm that the presence of CNCs does not seem to affect the health of immortalised chondrocytes (TC28a2) that remain viable over a period of seven days post-encapsulation. Taken together, our results indicate a favourable effect of the CNCs on the rheological and biocompatibility properties of alginate hydrogels, opening up new perspectives for the application of CNCs in the formulation of bioinks for extrusion-based bioprinting.
引用
收藏
页数:15
相关论文
共 51 条
[1]  
AULTHOUSE AL, 1989, IN VITRO CELL DEV B, V25, P659
[2]   Degradation of partially oxidized alginate and its potential application for tissue engineering [J].
Bouhadir, KH ;
Lee, KY ;
Alsberg, E ;
Damm, KL ;
Anderson, KW ;
Mooney, DJ .
BIOTECHNOLOGY PROGRESS, 2001, 17 (05) :945-950
[3]  
Boularaoui Selwa, 2020, Bioprinting, V20, pe00093, DOI [10.1016/j.bprint.2020.e00093, 10.1016/j.bprint.2020.e00093]
[4]   Biphasic Hydrogels Integrating Mineralized and Anisotropic Features for Interfacial Tissue Engineering [J].
Carmen Echave, Mari ;
Domingues, Rui M. A. ;
Gomez-Florit, Manuel ;
Luis Pedraz, Jose ;
Reis, Rui L. ;
Orive, Gorka ;
Gomes, Manuela E. .
ACS APPLIED MATERIALS & INTERFACES, 2019, 11 (51) :47771-47784
[5]   Widespread changes in transcriptome profile of human mesenchymal stem cells induced by two-dimensional nanosilicates [J].
Carrow, James K. ;
Cross, Lauren M. ;
Reese, Robert W. ;
Jaiswal, Manish K. ;
Gregory, Carl A. ;
Kaunas, Roland ;
Singh, Irtisha ;
Gaharwar, Akhilesh K. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018, 115 (17) :E3905-E3913
[6]   Effects of extracellular matrix viscoelasticity on cellular behaviour [J].
Chaudhuri, Ovijit ;
Cooper-White, Justin ;
Janmey, Paul A. ;
Mooney, David J. ;
Shenoy, Vivek B. .
NATURE, 2020, 584 (7822) :535-546
[7]   Printing Structurally Anisotropic Biocompatible Fibrillar Hydrogel for Guided Cell Alignment [J].
Chen, Zhengkun ;
Khuu, Nancy ;
Xu, Fei ;
Kheiri, Sina ;
Yakavets, Ilya ;
Rakhshani, Faeze ;
Morozova, Sofia ;
Kumacheva, Eugenia .
GELS, 2022, 8 (11)
[8]   Nanoengineered Ionic-Covalent Entanglement (NICE) Bioinks for 3D Bioprinting [J].
Chimene, David ;
Peak, Charles W. ;
Gentry, James L. ;
Carrow, James K. ;
Cross, Lauren M. ;
Mondragon, Eli ;
Cardoso, Guinea B. ;
Kaunas, Roland ;
Gaharwar, Akhilesh K. .
ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (12) :9957-9968
[9]   Bio-ink properties and printability for extrusion printing living cells [J].
Chung, Johnson H. Y. ;
Naficy, Sina ;
Yue, Zhilian ;
Kapsa, Robert ;
Quigley, Anita ;
Moulton, Simon E. ;
Wallace, Gordon G. .
BIOMATERIALS SCIENCE, 2013, 1 (07) :763-773
[10]   Geometric confinement is required for recovery and maintenance of chondrocyte phenotype in alginate [J].
Cooke M.E. ;
Pearson M.J. ;
Moakes R.J.A. ;
Weston C.J. ;
Davis E.T. ;
Jones S.W. ;
Grover L.M. .
APL Bioengineering, 2017, 1 (01)