Investigating comprehensive effects of depuration salinity and duration on posterior anhydrous living-preservation of Pacific oyster (Crassostrea gigas)

Depuration and anhydrous living-preservation are two important and consecutive steps during the circulation of Pacific oyster (Crassostrea gigas), and two main factors in depuration, salinity and duration, are pivotal to posterior anhydrous living-preservation of C. gigas. In this work, the comprehensive effects of depuration salinity (26-38 g/L) and duration (0-72 h) on anhydrous living-preservation at 4 degree celsius for 7 days were investigated in regard of mortality, biochemical indexes (fatty acids profile analysis, glycogen) and proteome changes as well. The results showed that the mortality of C. gigas increased obviously with 72 h depuration and especially with 20 % salinity fluctuation, concomitantly accompanying metabolism disorder. Furthermore, alterations in salinity and duration resulted in 381 different expression proteins (DEPs), which were gotten more involved in the pathways related to amino acid metabolism. Taken together, it was suggested that duration < 48 h and salinity fluctuations lower than 10 % were the preferred conditions for anhydrous living-preservation.