Rational Design of pH-Independent and High-Fidelity Near-Infrared Tunable Fluorescent Probes for Tracking Leucine Aminopeptidase In Vivo

被引:10
作者
Chen, Yu-Jin [1 ]
Zhang, Hong [1 ]
Liu, Yan-Zhao [1 ]
Shi, Lei [1 ]
Xiang, Fei-Fan [1 ]
Lin, Ru-De [1 ]
Liu, Yan-Hong [1 ]
Chen, Shan-Yong [1 ]
Yu, Xiao-Qi [1 ]
Li, Kun [1 ]
机构
[1] Sichuan Univ, Coll Chem, Key Lab Green Chem & Technol, Minist Educ, 29 Wangjiang Rd, Chengdu 610064, Peoples R China
基金
中国国家自然科学基金;
关键词
pH-independent; high-fidelity; fluorescencesignals; rhodamine; leucine aminopeptidase; LIVER-INJURY; CANCER; MODEL; LAP;
D O I
10.1021/acssensors.3c00470
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Accurate detection of target analytes and generationof high-fidelityfluorescence signals are particularly critical in life sciences andclinical diagnostics. However, the majority of current NIR-I fluorescentprobes are vulnerable to pH effects resulting in signal distortion.In this work, a series of fluorescence-tunable and pH-independentprobes are reported by combining optically tunable groups of unsymmetricSi-rhodamines and introducing the methoxy instead of the spiro ringon the benzene ring at position 9. To validate the concept, the leucineaminopeptidase response site was introduced into Si-2,6OMe-NH ( 2 ) with the best optical properties to synthesize Si-LAP for monitoring the intrahepatic LAP in vivo. Therefore,the design approach may provide a new and practical strategy for designinginnovative functional fluorescent probes and generating high-stabilityand high-fidelity fluorescent signals.
引用
收藏
页码:2359 / 2367
页数:9
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