Acquisition of a novel conjugative multidrug-resistant hypervirulent plasmid leads to hypervirulence in clinical carbapenem-resistant Klebsiella pneumoniae strains

被引：4

作者：

Li, Gong ^{[1
,2
]}

Jia, Ling ^{[1
,2
]}

Wan, Lei ^{[1
,2
]}

Xia, Lijuan ^{[1
,2
]}

Gao, Ang ^{[1
,2
]}

Yang, Runshi ^{[3
]}

Sun, Ruanyang ^{[1
,2
]}

Wang, Minge ^{[1
,2
]}

Du, Juan ^{[1
,2
]}

Lian, Xinlei ^{[1
,2
]}

Zhang, Rongmin ^{[1
,2
]}

Fang, Liangxing ^{[1
,2
]}

Liao, Xiaoping ^{[1
,2
]}

Liu, Yahong ^{[1
,2
]}

Liu, Bao-Tao ^{[4
]}

Sun, Jian ^{[1
,2
]}

机构：

[1] South China Agr Univ, State Key Lab Anim Dis Control & Prevent, Guangzhou, Peoples R China

[2] South China Agr Univ, Guangdong Prov Key Lab Vet Pharmaceut Dev & Safety, Guangzhou, Peoples R China

[3] Zhejiang Univ, Sch Med, Dept Pathogen Biol & Microbiol, Hangzhou, Peoples R China

[4] Qingdao Agr Univ, Coll Vet Med, Qingdao, Peoples R China

来源：

MLIFE | 2023年 / 2卷 / 03期

基金：

中国国家自然科学基金;

关键词：

conjugative virulence plasmid; CR-HvKP; hybrid plasmid; Klebsiella pneumoniae; ST23; HIGH PREVALENCE; CHICKEN MEAT;

D O I：

10.1002/mlf2.12086

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The co-occurrence of plasmid-mediated multidrug resistance and hypervirulence in epidemic carbapenem-resistant Klebsiella pneumoniae has emerged as a global public health issue. In this study, an ST23 carbapenem-resistant hypervirulent K. pneumoniae (CR-HvKP) strain VH1-2 was identified from cucumber in China and harbored a novel hybrid plasmid pVH1-2-VIR. The plasmid pVH1-2-VIR carrying both virulence and multidrug-resistance (MDR) genes was likely generated through the recombination of a virulence plasmid and an IncFIIK conjugative MDR plasmid in clinical ST23 18622 isolated from a sputum sample. The plasmid pVH1-2-VIR exhibited the capacity for transfer to the clinical ST11 carbapenem-resistant K. pneumoniae (CRKP) strain via conjugation assay. Acquisition of pVH1-2-VIR plasmid directly converted a CRKP into CR-HvKP strain characterized by hypermucoviscosity, heightened virulence for Galleria mellonella larvae, and increased colonization ability in the mouse intestine. The emergence of such a hybrid plasmid may expedite the spread of CR-HvKP strains, posing a significant risk to human health.

引用

页码：317 / 327

页数：11

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