Acquisition of a novel conjugative multidrug-resistant hypervirulent plasmid leads to hypervirulence in clinical carbapenem-resistant Klebsiella pneumoniae strains

被引：4

作者：

Li, Gong ^{[1
,2
]}

Jia, Ling ^{[1
,2
]}

Wan, Lei ^{[1
,2
]}

Xia, Lijuan ^{[1
,2
]}

Gao, Ang ^{[1
,2
]}

Yang, Runshi ^{[3
]}

Sun, Ruanyang ^{[1
,2
]}

Wang, Minge ^{[1
,2
]}

Du, Juan ^{[1
,2
]}

Lian, Xinlei ^{[1
,2
]}

Zhang, Rongmin ^{[1
,2
]}

Fang, Liangxing ^{[1
,2
]}

Liao, Xiaoping ^{[1
,2
]}

Liu, Yahong ^{[1
,2
]}

Liu, Bao-Tao ^{[4
]}

Sun, Jian ^{[1
,2
]}

机构：

[1] South China Agr Univ, State Key Lab Anim Dis Control & Prevent, Guangzhou, Peoples R China

[2] South China Agr Univ, Guangdong Prov Key Lab Vet Pharmaceut Dev & Safety, Guangzhou, Peoples R China

[3] Zhejiang Univ, Sch Med, Dept Pathogen Biol & Microbiol, Hangzhou, Peoples R China

[4] Qingdao Agr Univ, Coll Vet Med, Qingdao, Peoples R China

来源：

MLIFE | 2023年 / 2卷 / 03期

基金：

中国国家自然科学基金;

关键词：

conjugative virulence plasmid; CR-HvKP; hybrid plasmid; Klebsiella pneumoniae; ST23; HIGH PREVALENCE; CHICKEN MEAT;

D O I：

10.1002/mlf2.12086

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The co-occurrence of plasmid-mediated multidrug resistance and hypervirulence in epidemic carbapenem-resistant Klebsiella pneumoniae has emerged as a global public health issue. In this study, an ST23 carbapenem-resistant hypervirulent K. pneumoniae (CR-HvKP) strain VH1-2 was identified from cucumber in China and harbored a novel hybrid plasmid pVH1-2-VIR. The plasmid pVH1-2-VIR carrying both virulence and multidrug-resistance (MDR) genes was likely generated through the recombination of a virulence plasmid and an IncFIIK conjugative MDR plasmid in clinical ST23 18622 isolated from a sputum sample. The plasmid pVH1-2-VIR exhibited the capacity for transfer to the clinical ST11 carbapenem-resistant K. pneumoniae (CRKP) strain via conjugation assay. Acquisition of pVH1-2-VIR plasmid directly converted a CRKP into CR-HvKP strain characterized by hypermucoviscosity, heightened virulence for Galleria mellonella larvae, and increased colonization ability in the mouse intestine. The emergence of such a hybrid plasmid may expedite the spread of CR-HvKP strains, posing a significant risk to human health.

引用

页码：317 / 327

页数：11

共 50 条

[21] Risk Factors for the Acquisition of Nosocomial Infection with Carbapenem-Resistant Klebsiella pneumoniae [J].

Wu, Dandan ;

Cai, Jiachang ;

Liu, Jin .

SOUTHERN MEDICAL JOURNAL, 2011, 104 (02) :106-110

[22] Hypervirulent carbapenem-resistant Klebsiella pneumoniae causing highly fatal meningitis in southeastern China [J].

Huang, Na ;

Jia, Huaiyu ;

Zhou, Beibei ;

Zhou, Cui ;

Cao, Jianming ;

Liao, Wenli ;

Liu, Shixing ;

Wang, Lingbo ;

Chen, Liqiong ;

Chen, Lijiang ;

Zhou, Tieli ;

Ye, Jianzhong .

FRONTIERS IN PUBLIC HEALTH, 2022, 10

[23] The mechanisms of resistance, epidemiological characteristics, and molecular evolution of carbapenem-resistant hypervirulent Klebsiella pneumoniae [J].

Wang, Qun ;

Ye, Mei-Yi ;

Hong, Chi ;

Li, Zu-Pin ;

Lin, Lei .

LABORATORY MEDICINE, 2025, 56 (04) :323-335

[24] Carbapenem-resistant hypervirulent ST23 Klebsiella pneumoniae with a highly transmissible dual-carbapenemase plasmid in Chile [J].

Galvez-Silva, Matias ;

Arros, Patricio ;

Berrios-Pasten, Camilo ;

Villamil, Aura ;

Rodas, Paula I. ;

Araya, Ingrid ;

Iglesias, Rodrigo ;

Araya, Pamela ;

Hormazabal, Juan C. ;

Bohle, Constanza ;

Chen, Yahua ;

Gan, Yunn-Hwen ;

Chavez, Francisco P. ;

Lagos, Rosalba ;

Marcoleta, Andres E. .

BIOLOGICAL RESEARCH, 2024, 57 (01)

[25] Epidemiological study of carbapenem-resistant Klebsiella pneumoniae [J].

Lin, Di ;

Chen, Jian ;

Yang, Yan ;

Cheng, Jun ;

Sun, Changgui .

OPEN MEDICINE, 2018, 13 (01) :460-466

[26] Tracking clonal and plasmid transmission in colistin- and carbapenem-resistant Klebsiella pneumoniae [J].

Akintayo, Ifeoluwa ;

Siroglavic, Marko ;

Frolova, Daria ;

Silva, Mabel Budia ;

Grundmann, Hajo ;

Iqbal, Zamin ;

Budimir, Ana ;

Reuter, Sandra .

MSYSTEMS, 2025, 10 (02)

[27] Clinical evolution of ST11 carbapenem resistant and hypervirulent Klebsiella pneumoniae [J].

Xie, Miaomiao ;

Yang, Xuemei ;

Xu, Qi ;

Ye, Lianwei ;

Chen, Kaichao ;

Zheng, Zhiwei ;

Dong, Ning ;

Sun, Qiaoling ;

Shu, Lingbin ;

Gu, Danxia ;

Chan, Edward Wai-Chi ;

Zhang, Rong ;

Chen, Sheng .

COMMUNICATIONS BIOLOGY, 2021, 4 (01)

[28] Analysis of an IncR plasmid carried by carbapenem-resistant Klebsiella pneumoniae: A survey of swine Klebsiella pneumoniae in Jilin Province [J].

Qi, Yu ;

Xue, Jun-Ze ;

Li, Shuang-shuang ;

Elken, Emad Mohammed ;

Haqmal, M. Aman ;

Li, Xue-Song ;

Xu, Guan-Yi ;

Kong, Ling-Cong ;

Ma, Hong-Xia .

JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2023, 34 :83-90

[29] Occurrence of carbapenem-resistant hypervirulent Klebsiella pneumoniae in oysters in Egypt: a significant public health issue [J].

Mohammed, Rahma ;

Nader, Sara M. ;

Hamza, Dalia A. ;

Sabry, Maha A. .

ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS, 2024, 23 (01)

[30] Evidence of hypervirulent carbapenem-resistant Klebsiella pneumoniae in cats with urinary affections and associated humans in Egypt [J].

Hashem, Sarah M. ;

Abdel-Kader, Fatma ;

Ismael, Elshaimaa ;

Hassan, Ayah M. ;

Farouk, Manar M. ;

Elhariri, Mahmoud ;

Elhelw, Rehab .

SCIENTIFIC REPORTS, 2025, 15 (01)

← 1 2 3 4 5 →