The effect of polystyrene nanoplastics on arsenic-induced apoptosis in HepG2 cells

被引：4

作者：

He, Lei ^{[1
]}

Lu, Zifan ^{[1
]}

Zhang, Yuanyuan ^{[2
]}

Yan, Linhong ^{[2
]}

Ma, Lihua ^{[2
]}

Dong, Xiaoling ^{[2
]}

Wu, Zijie ^{[1
]}

Dai, Zhenqing ^{[1
,2
,3
]}

Tan, Baoyi ^{[1
]}

Sun, Ruikun ^{[1
]}

Sun, Shengli ^{[1
]}

Li, Chengyong ^{[1
,2
,3
]}

机构：

[1] Guangdong Ocean Univ, Sch Chem & Environm, Zhanjiang 524088, Peoples R China

[2] Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China

[3] Guangdong Ocean Univ, Guangdong Prov Key Lab Intelligent Equipment South, Zhanjiang 524088, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2024年 / 269卷

关键词：

Arsenic; Polystyrene microplastics; HepG2; cells; Apoptosis; DEPENDENT ANION CHANNEL; DNA-REPLICATION; CASPASE-3; DEATH; PHOSPHORYLATION; INHIBITION; PARTICLES; PROTEIN; METALS; XIAP;

D O I：

10.1016/j.ecoenv.2023.115814

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Microplastics are detrimental to the environment. However, the combined effects of microplastics and arsenic (As) remain unclear. In this study, we investigated the combined effects of polystyrene (PS) microplastics and As on HepG2 cells. The results showed that PS microplastics 20, 50, 200, and 500 nm in size were taken up by HepG2 cells, causing a decrease in cellular mitochondrial membrane potential. The results of lactate dehydrogenase release and flow cytometry showed that PS microplastics, especially those of 50 nm, enhanced As-induced apoptosis. In addition, transcriptome analysis revealed that TP53, AKT1, CASP3, ACTB, BCL2L1, CASP8, XIAP, MCL1, NFKBIA, and CASP7 were the top 10 hub genes for PS that enhanced the role of As in HepG2 cell apoptosis. Our results suggest that nano-PS enhances As-induced apoptosis. Furthermore, this study is important for a better understanding of the role of microplastics in As-induced hepatotoxicity.

引用

页数：9

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