JANUS: an open-source 3D printable perfusion bioreactor and numerical model-based design strategy for tissue engineering

被引:1
作者
Meneses, Joao [1 ,2 ]
Fernandes, Sofia R. [2 ]
Silva, Joao C. [3 ,4 ,5 ]
Ferreira, Frederico Castelo [3 ,4 ,5 ]
Alves, Nuno [1 ,6 ,7 ]
Pascoal-Faria, Paula [1 ,7 ,8 ]
机构
[1] Polytech Leiria, Ctr Rapid & Sustainable Prod Dev, Marinha Grande, Portugal
[2] Univ Lisbon, Inst Biofis & Engn Biomed, Fac Ciencias, Lisbon, Portugal
[3] Univ Lisbon, Dept Bioengn, Inst Super Tecn, Lisbon, Portugal
[4] Univ Lisbon, iBB Inst Bioengn & Biosci, Inst Super Tecn, Lisbon, Portugal
[5] Univ Lisbon, I4HB Inst Hlth & Bioecon, Associate Lab, Inst Super Tecn, Lisbon, Portugal
[6] Polytech Leiria, Dept Mech Engn, Sch Technol & Management, Leiria, Portugal
[7] Associate Lab Adv Prod & Intelligent Syst ARISE, P-4050313 Porto, Portugal
[8] Polytech Leiria, Sch Technol & Management, Dept Math, Leiria, Portugal
关键词
bioreactor; modeling; design; capacitive-coupled; perfusion flow; multimodal stimulation; tissue engineering; finite element analysis; BONE-TISSUE; FLUID-FLOW; OSTEOGENESIS; SCAFFOLDS; STIMULATION; TRANSPORT; SYSTEM; CELLS; FIELD;
D O I
10.3389/fbioe.2023.1308096
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bioreactors have been employed in tissue engineering to sustain longer and larger cell cultures, managing nutrient transfer and waste removal. Multiple designs have been developed, integrating sensor and stimulation technologies to improve cellular responses, such as proliferation and differentiation. The variability in bioreactor design, stimulation protocols, and cell culture conditions hampered comparison and replicability, possibly hiding biological evidence. This work proposes an open-source 3D printable design for a perfusion bioreactor and a numerical model-driven protocol development strategy for improved cell culture control. This bioreactor can simultaneously deliver capacitive-coupled electric field and fluid-induced shear stress stimulation, both stimulation systems were validated experimentally and in agreement with numerical predictions. A preliminary in vitro validation confirmed the suitability of the developed bioreactor to sustain viable cell cultures. The outputs from this strategy, physical and virtual, are openly available and can be used to improve comparison, replicability, and control in tissue engineering applications.
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收藏
页数:16
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