The Protein Fusion Strategy Facilitates the Structure Determination of Small Membrane Proteins by Cryo-EM

被引：4

作者：

Cui, Wenhao ^{[1
]}

Niu, Yange ^{[1
]}

Chen, Lei ^{[1
,2
]}

机构：

[1] Peking Univ, Beijing Key Lab Cardiometab Mol Med, Coll Future Technol, State Key Lab Membrane Biol,Inst Mol Med, Beijing 100871, Peoples R China

[2] Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China

来源：

BIOCHEMISTRY | 2023年 / 62卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Medical imaging;

D O I：

10.1021/acs.biochem.2c00319

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite the resolution revolution of cryo-EM, structures of small membrane proteins (<80 kDa) are still understudied. These proteins are notoriously reluctant to structure determination by single-particle cryo-EM. Protein fusion might represent a plausible strategy to overcome such difficulties. This Perspective enumerates recent exemplary progress and discusses the future potential of the protein fusion strategy.

引用

页码：196 / 200

页数：5

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