Hydrogel-encapsulation to enhance bacterial diagnosis of colon inflammation

被引：20

作者：

Aghlara-Fotovat, Samira ^{[1
]}

Musteata, Elena ^{[2
]}

Doerfert, Michael D. ^{[1
]}

Baruch, Moshe ^{[1
]}

Levitan, Maya ^{[1
]}

Tabor, Jeffrey J. ^{[1
,2
,3
]}

Veiseh, Omid ^{[1
]}

机构：

[1] Rice Univ, Dept Bioengn, Houston, TX 77005 USA

[2] Rice Univ, Syst Synthet & Phys Biol, Houston, TX USA

[3] Rice Univ, Dept Biosci, Houston, TX USA

来源：

BIOMATERIALS | 2023年 / 301卷

基金：

美国国家科学基金会;

关键词：

Hydrogel encapsulation; Synthetic biology; Diagnostic bacteria; Colitis; GUT MICROBIOME; CROHNS-DISEASE; BOWEL-DISEASE; CELLS; IMMUNOMODULATORS; AZATHIOPRINE; METABOLITES; THERAPY; RESPOND; SURGERY;

D O I：

10.1016/j.biomaterials.2023.122246

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Bacteria can be genetically programmed to sense and report the presence of disease biomarkers in the gastro-intestinal (GI) tract. However, diagnostic bacteria are typically delivered via oral administration of liquid cul-tures, resulting in poor survival and high dispersal in vivo. These limitations confound recovery and analysis of engineered bacteria from GI or stool samples. Here, we demonstrate that encapsulating bacteria inside of alginate core-shell particles enables robust survival, containment, and diagnostic function in vivo. We demonstrate these benefits by encapsulating a strain engineered to report the presence of the biomarker thiosulfate via fluorescent protein expression in order to diagnose dextran sodium sulfate-induced colitis in rats. Hydrogel-encapsulated bacteria engineered to sense and respond to physiological stimuli should enable minimally invasive moni-toring of a wide range of diseases and have applications as next-generation smart therapeutics.

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页数：13

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