A genome-wide landscape of mRNAs, miRNAs, lncRNAs, and circRNAs of skeletal muscles during dietary restriction in Mongolian horses

被引:3
作者
Bou, Tugeqin
Ding, Wenqi
Liu, Huiying
Gong, Wendian
Jia, Zijie
Dugarjaviin, Manglai
Bai, Dongyi
机构
[1] Inner Mongolia Agr Univ, Minist Agr & Rural Affairs, Key Lab Equus Germplasm Innovat Coconstruct Minis, Hohhot 010018, Peoples R China
[2] Inner Mongolia Agr Univ, Inner Mongolia Key Lab Equine Genet Breeding & Re, Hohhot 010018, Peoples R China
[3] Inner Mongolia Agr Univ, Coll Anim Sci, Equus Res Ctr, Hohhot 010018, Peoples R China
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2023年 / 46卷
基金
中国国家自然科学基金;
关键词
Skeletal muscle; Dietary restriction; Transcriptomics; Non-coding RNA; Expression profile; ENERGY-METABOLISM; EXERCISE; EXPRESSION; INTENSITY; FIBERS;
D O I
10.1016/j.cbd.2023.101084
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The proportion of different muscle fibers is essential for the horse breed's aptitude for athletic activities. Adaptation of locomotor muscle is correlated with altered physiologic conditions. To investigate the adaptive changes of muscle fiber phenotype and transcriptome in horse skeletal muscle during dietary restriction (DR). The muscle fiber type distribution and deep RNA-seq analysis of detecting differentially expressed mRNAs (DEGs), miRNA (DEMIRs), lncRNAs (DELs), circRNAs (DECs), and their function analysis were investigated in gluteus medius muscle of Mongolian horses during DR. A total of 1433 DEGs, 5 DEMIRs, 329 DELs, and 53 DECs were identified. Differing from non-uniform muscle fiber type changing, functional enrichment analysis showed that most downregulated DEGs were associated in muscle contraction, fuel energy metabolism, and protein balance. Linkages between non-coding RNA and mRNA landscape were detected from their functional changes. Our study provides new insights into the expressional changes of mRNA and non-coding RNA in horse skeletal muscles during DR, which might improve our understanding of the molecular mechanisms regulating muscle adaption during DR for racing horses.
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页数:10
相关论文
共 41 条
[1]   Predicting effective microRNA target sites in mammalian mRNAs [J].
Agarwal, Vikram ;
Bell, George W. ;
Nam, Jin-Wu ;
Bartel, David P. .
ELIFE, 2015, 4
[2]   Gene expression of proteolytic systems and growth regulators of skeletal muscle in horses with myopathy associated with pituitary pars intermedia dysfunction [J].
Aleman, Monica ;
Nieto, Jorge E. .
AMERICAN JOURNAL OF VETERINARY RESEARCH, 2010, 71 (06) :664-670
[3]   Proteomics Can Help to Gain Insights into Metabolic Disorders According to Body Reserve Availability [J].
Bertile, F. ;
Raclot, T. .
CURRENT MEDICINAL CHEMISTRY, 2008, 15 (25) :2545-2558
[4]   Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1 [J].
Bodine, Sue C. ;
Baehr, Leslie M. .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2014, 307 (06) :E469-E484
[5]  
Bouguerra L., 2017, BIOL RHYTHM RES, P1
[6]   High intensity exercise increases expression of matrix metalloproteinases in fast skeletal muscle fibres [J].
Carmeli, E ;
Moas, M ;
Lennon, S ;
Powers, SK .
EXPERIMENTAL PHYSIOLOGY, 2005, 90 (04) :613-619
[7]   TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data [J].
Chen, Chengjie ;
Chen, Hao ;
Zhang, Yi ;
Thomas, Hannah R. ;
Frank, Margaret H. ;
He, Yehua ;
Xia, Rui .
MOLECULAR PLANT, 2020, 13 (08) :1194-1202
[8]   Plasticity of Mitochondrial Translation [J].
Dennerlein, Sven ;
Wang, Cong ;
Rehling, Peter .
TRENDS IN CELL BIOLOGY, 2017, 27 (10) :712-721
[9]   PGC-1α encoded by the PPARGC1A gene regulates oxidative energy metabolism in equine skeletal muscle during exercise [J].
Eivers, S. S. ;
McGivney, B. A. ;
Gu, J. ;
MacHugh, D. E. ;
Katz, L. M. ;
Hill, E. W. .
ANIMAL GENETICS, 2012, 43 (02) :153-162
[10]   MicroRNA targets in Drosophila [J].
Anton J Enright ;
Bino John ;
Ulrike Gaul ;
Thomas Tuschl ;
Chris Sander ;
Debora S Marks .
Genome Biology, 5 (1)