Molecular Identification of UDP-Sugar-Dependent Glycosyltransferase and Acyltransferase Involved in the Phenylethanoid Glycoside Biosynthesis Induced by Methyl Jasmonate in Sesamum indicum L.

被引：8

作者：

Fuji, Yushiro ^{[1
,2
]}

Uchida, Kai ^{[1
]}

Akashi, Tomoyoshi ^{[3
]}

Ohtsuki, Takashi ^{[2
]}

Matsufuji, Hiroshi ^{[2
]}

Hirai, Masami Yokota ^{[1
,4
]}

机构：

[1] RIKEN, Ctr Sustainable Resource Sci, Yokohama 2300045, Japan

[2] Nihon Univ, Coll Bioresource Sci, Dept Food Sci & Technol, Fujisawa, Kanagawa 2520880, Japan

[3] Nihon Univ, Coll Bioresource Sci, Dept Appl Biol Sci, Fujisawa, Kanagawa 2520880, Japan

[4] Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Biosci, Nagoya 4648601, Japan

来源：

PLANT AND CELL PHYSIOLOGY | 2023年 / 64卷 / 07期

关键词：

Acteoside; Acyltransferase; Biosynthesis; Glucosyltransferase; Phenylethanoid glycoside; Sesamum indicum L; UDP-sugar-dependent glycosyltransferase; SUSPENSION-CULTURES; ACTEOSIDE; GLUCOSYLTRANSFERASE; OVEREXPRESSION; IMPROVEMENT; CLONING; GENES;

D O I：

10.1093/pcp/pcad053

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Sesame (Sesamum indicum L.) plants contain large amounts of acteoside, a typical phenylethanoid glycoside (PhG) that exhibits various pharmacological activities. Although there is increasing interest in the biosynthesis of PhGs for improved production, the pathway remains to be clarified. In this study, we established sesame-cultured cells and performed transcriptome analysis of methyl jasmonate (MeJA)-treated cultured cells to identify enzyme genes responsible for glucosylation and acylation in acteoside biosynthesis. Among the genes annotated as UDP-sugar-dependent glycosyltransferase (UGT) and acyltransferase (AT), 34 genes and one gene, respectively, were upregulated by MeJA in accordance with acteoside accumulation. Based on a phylogenetic analysis, five UGT genes (SiUGT1-5) and one AT gene (SiAT1) were selected as candidate genes involved in acteoside biosynthesis. Additionally, two AT genes (SiAT2-3) were selected based on sequence identity. Enzyme assays using recombinant SiUGT proteins revealed that SiUGT1, namely, UGT85AF10, had the highest glucosyltransferase activity among the five candidates against hydroxytyrosol to produce hydroxytyrosol 1-O-glucoside. SiUGT1 also exhibited glucosyltransferase activity against tyrosol to produce salidroside (tyrosol 1-O-glucoside). SiUGT2, namely, UGT85AF11, had similar activity against hydroxytyrosol and tyrosol. Enzyme assay using the recombinant SiATs indicated that SiAT1 and SiAT2 had activity transferring the caffeoyl group to hydroxytyrosol 1-O-glucoside and salidroside (tyrosol 1-O-glucoside) but not to decaffeoyl-acteoside. The caffeoyl group was attached mainly at the 4-position of glucose of hydroxytyrosol 1-O-glucoside, followed by attachment at the 6-position and the 3-position of glucose. Based on our results, we propose an acteoside biosynthetic pathway induced by MeJA treatment in sesame.

引用

页码：716 / 728

页数：13