Doxycycline treatment modulates the immune response of tilapia and controls Aeromonas hydrophila infection

被引:0
作者
de Oliveira, Susana Luporini [1 ]
da Costa, Camila Carlino [1 ]
Aracati, Mayumi Fernanda [1 ]
Rodrigues, Leticia Franchin [1 ]
Montassier, Helio Jose [1 ]
Conde, Gabriel [2 ]
Rodrigues, Romario Alves [3 ]
Charlie-Silva, Ives [4 ]
Clissa, Patricia Bianca [5 ]
Pereira, Luiz Arthur Malta [6 ]
Belo, Marco Antonio de Andrade [6 ,7 ]
机构
[1] Dept Patol Reprod & Saude Un, Dept Patol Reprod & Saude Unica, FCAV, Jaboticabal, SP, Brazil
[2] Univ Araraquara UNIARA, Araraquara, SP, Brazil
[3] Dept Microbiol, UNESP, FCAV, Jaboticabal, SP, Brazil
[4] UNESP, IQ UNESP, Araraquara, SP, Brazil
[5] Inst Butantan, Sao Paulo, SP, Brazil
[6] Univ Brazil, Lab Farmacol & Toxicol Anim, Descalvado, SP, Brazil
[7] Univ Brazil, pq Univ, Campus Descalvado,Ave Hilario da Silva Passos 950, BR-13690000 Descalvado, Brazil
关键词
Cichlids; Fish diseases; Aeromonosis; Tetracyclines; Therapeutic efficacy; Immune response; OREOCHROMIS-NILOTICUS; NILE TILAPIA; HEMATOLOGY; PARAMETERS; STRESS; PACUS;
D O I
10.1016/j.aquaculture.2023.740504
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The objective of this study was to evaluate the immunopharmacological activity of doxycycline, administered orally in Nile tilapia (Oreochromis niloticus), during experimental infection by Aeromonas hydrophila, through the correlation between therapeutic efficacy and fish immune response. 160 Nile tilapia (+/- 100 g) from the same spawning were distributed in 16 tanks (100 L, n = 10), constituting four treatments: control (infected and untreated); Infected and Treated with 20, 40 and 80 mg of doxycycline/Kg of b.w. Control fish and treated with the lowest dose of doxycycline (20 mg) exhibited typical signs of aeromonosis, including exophthalmos, fin erosion, hemorrhagic septicemia, corneal opacity and ascites. No clinical signs were observed in animals treated with 40 and 80 mg of doxycycline. There was a dose -response effect to the treatment with this tetracycline with relative percentage of survival (RPS) of 60, 73.3 and 93.3% for the treatments with 20, 40 and 80 mg/kg of b.w. when compared to control animals, as well as increased in the production of antibodies anti -A. hydrophila. Doxycycline treatment decreased monocytosis and reactive oxygen species (ROS) production observed in tilapia during A. hydrophila infection. Therefore, doxycycline administered orally to O. niloticus revealed the potential of this antimicrobial for use in tilapia farming, providing convincing results of the therapeutic efficacy against A. hydrophila. These results confirm the hypothesis that the antimicrobial action of doxycycline favored the defense mechanisms of tilapia treated mainly at a dose of 80 mg/kg. Although the exact mechanism of increased antibody response remains to be elucidated, the present observations demonstrate important practical significance for the sanitary management of tilapia farming.
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页数:11
相关论文
共 47 条
[21]   IκBα phosphorylation and associated NF-κB activation are essential events in lymphocyte activation, proliferation, and anti-bacterial adaptive immune response of Nile tilapia [J].
Li, Cheng ;
Yu, Junkun ;
Ai, Kete ;
Li, Huiying ;
Zhang, Yu ;
Zhao, Tianyu ;
Wei, Xiumei ;
Yang, Jialong .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 2020, 103
[22]   Innate immunity of fish (overview) [J].
Magnadóttir, B .
FISH & SHELLFISH IMMUNOLOGY, 2006, 20 (02) :137-151
[23]   Determination of Microbial Diversity of Aeromonas Strains on the Basis of Multilocus Sequence Typing, Phenotype, and Presence of Putative Virulence Genes [J].
Martino, Maria Elena ;
Fasolato, Luca ;
Montemurro, Filomena ;
Rosteghin, Marina ;
Manfrin, Amedeo ;
Patarnello, Tomaso ;
Novelli, Enrico ;
Cardazzo, Barbara .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2011, 77 (14) :4986-5000
[24]   Haematological changes in Nile tilapia experimentally infected with Enterococcus sp. [J].
Martins, M. L. ;
Mourino, J. L. P. ;
Amaral, G., V ;
Vieira, F. N. ;
Dotta, G. ;
Jatoba, A. M. B. ;
Pedrotti, F. S. ;
Jeronimo, G. T. ;
Buglione-Neto, C. C. ;
Pereira-Jr, G. .
BRAZILIAN JOURNAL OF BIOLOGY, 2008, 68 (03) :657-661
[25]  
Moraes AC., 2022, CLEAN CHEM ENG, V01, P100005, DOI [10.1016/j.clce.2022.100005, DOI 10.1016/J.CLCE.2022.100005]
[26]  
Nafiqoh Nunak, 2022, Hayati Journal of Biosciences, V29, P137, DOI 10.4308/hjb.29.2.137-145
[27]  
Nakamura Y., 1982, Fish Pathology, V17, P67
[28]   A NEW BLOOD DILUENT FOR COUNTING THE ERYTHROCYTES AND LEUCOCYTES OF THE CHICKEN [J].
NATT, MP ;
HERRICK, CA .
POULTRY SCIENCE, 1952, 31 (04) :735-738
[29]   Is Aeromonas hydrophila the dominant motile Aeromonas species that causes disease outbreaks in aquaculture production in the Zhejiang Province of China? [J].
Nielsen, ME ;
Hoi, L ;
Schmidt, AS ;
Qian, D ;
Shimada, T ;
Shen, JY ;
Larsen, JL .
DISEASES OF AQUATIC ORGANISMS, 2001, 46 (01) :23-29
[30]  
Oliveira S.L., 2021, IJDDR, V11, P47914, DOI [10.37118/ ijdr.22149.06.2021, DOI 10.37118/IJDR.22149.06.2021, 10.37118/ijdr.22149.06.2021]