High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae

被引:3
作者
Stazzoni, Samuele [1 ]
Troisi, Marco [1 ]
Abbiento, Valentina [1 ,2 ]
Sala, Claudia [1 ]
Andreano, Emanuele [1 ]
Rappuoli, Rino [2 ,3 ]
机构
[1] Fdn Toscana Life Sci, Monoclonal Antibody Discovery Lab, Siena, Italy
[2] Univ Siena, Dept Biotechnol Chem & Pharm, Siena, Italy
[3] Fdn Biotecnopolo Siena, Siena, Italy
基金
欧洲研究理事会;
关键词
monoclonal antibodies; Neisseria gonorrhoeae; bacteria; antimicrobial resistance; therapeutics; high-throughput screening; EVOLUTION;
D O I
10.3389/fmicb.2023.1243427
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public health burden and the need to develop novel therapeutic and prophylactic tools is now a global priority. While high-throughput screening methods allowed rapid discovery of extremely potent monoclonal antibodies (mAbs) against viral pathogens, the field of bacteriology suffers from the lack of assays that allow efficient screening of large panels of samples. To address this point, we developed luminescence-based (L-ABA) and resazurin-based (R-ABA) antibody bactericidal assays that measure N. gonorrhoeae metabolic activity as a proxy of bacterial viability. Both L-ABA and R-ABA are applicable on the large scale for the rapid identification of bactericidal antibodies and were validated by conventional methods. Implementation of these approaches will be instrumental to the development of new medications and vaccines against N. gonorrhoeae and other bacterial pathogens to support the fight against antimicrobial resistance.
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页数:8
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