CyTOF protocol for immune monitoring of solid tumors from mouse models

被引：7

作者：

Shin, Sarah M. ^{[1
]}

Hernandez, Alexei ^{[1
]}

Coyne, Erin ^{[1
]}

Munjal, Kabeer ^{[1
]}

Gross, Nicole E. ^{[1
]}

Charmsaz, Soren ^{[1
]}

Yuan, Xuan ^{[1
,2
]}

Yang, Hongqui ^{[1
,2
]}

Ho, Won Jin ^{[1
,2
,3
]}

机构：

[1] Sidney Kimmel Comprehens Canc Ctr Johns Hopkins, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Baltimore, MD 21287 USA

[3] Johns Hopkins Univ, Convergence Inst, Baltimore, MD 21287 USA

来源：

STAR PROTOCOLS | 2023年 / 4卷 / 01期

关键词：

Antibody; Cancer; Flow Cytometry/Mass Cytometry; Immunology;

D O I：

10.1016/j.xpro.2022.101949

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects.For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021).1

引用

页数：27

共 11 条

[1] Isolation and adoptive transfer of innate lymphoid cells 2 to a recipient mouse model of PDAC [J].

Alam, Aftab ;

Comer, Sarah ;

Levanduski, Eric ;

Dey, Prasenjit .

STAR PROTOCOLS, 2022, 3 (03)

[2] Optimizing Flow Cytometric Analysis of Immune Cells in Samples Requiring Cryopreservation from Tumor-Bearing Mice [J].

Carlson, Peter M. ;

Mohan, Manasi ;

Patel, Ravi B. ;

Birstler, Jen ;

Nettenstrom, Lauren ;

Sheerar, Dagna ;

Fox, Kathryn ;

Rodriguez, Matthew ;

Hoefges, Anna ;

Hernandez, Reinier ;

Zahm, Chris ;

Kim, KyungMann ;

McNeel, Douglas G. ;

Weichert, Jamey ;

Morris, Zachary S. ;

Sondel, Paul M. .

JOURNAL OF IMMUNOLOGY, 2021, 207 (02) :720-734

[3] A global live cell barcoding approach for multiplexed mass cytometry profiling of mouse tumors [J].

Charmsaz, Soren ;

Gross, Nicole ;

Jaffee, Elizabeth ;

Ho, Won Jin .

JCI INSIGHT, 2021, 6 (07)

[4] Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer [J].

de la Fuente, Laura Rodriguez ;

Law, Andrew M. K. ;

Gallego-Ortega, David ;

Valdes-Mora, Fatima .

STAR PROTOCOLS, 2021, 2 (04)

[5] Systemic inhibition of PTPN22 augments anticancer immunity [J].

Ho, Won Jin ;

Croessmann, Sarah ;

Lin, Jianping ;

Phyo, Zaw H. ;

Charmsaz, Soren ;

Danilova, Ludmila ;

Mohan, Aditya A. ;

Gross, Nicole E. ;

Chen, Fangluo ;

Dong, Jiajun ;

Aggarwal, Devesh ;

Bai, Yunpeng ;

Wang, Janey ;

He, Jing ;

Leatherman, James M. ;

Yarchoan, Mark ;

Armstrong, Todd D. ;

Zaidi, Neeha ;

Fertig, Elana J. ;

Denny, Joshua C. ;

Park, Ben H. ;

Zhang, Zhong-Yin ;

Jaffee, Elizabeth M. .

JOURNAL OF CLINICAL INVESTIGATION, 2021, 131 (17)

[6] Multi-omic profiling of lung and liver tumor microenvironments of metastatic pancreatic cancer reveals site-specific immune regulatory pathways [J].

Ho, Won Jin ;

Erbe, Rossin ;

Danilova, Ludmila ;

Phyo, Zaw ;

Bigelow, Emma ;

Stein-O'Brien, Genevieve ;

Thomas, Dwayne L. ;

Charmsaz, Soren ;

Gross, Nicole ;

Woolman, Skylar ;

Cruz, Kayla ;

Munday, Rebecca M. ;

Zaidi, Neeha ;

Armstrong, Todd D. ;

Sztein, Marcelo B. ;

Yarchoan, Mark ;

Thompson, Elizabeth D. ;

Jaffee, Elizabeth M. ;

Fertig, Elana J. .

GENOME BIOLOGY, 2021, 22 (01)

[7] A Beginner's Guide to Analyzing and Visualizing Mass Cytometry Data [J].

Kimball, Abigail K. ;

Oko, Lauren M. ;

Bullock, Bonnie L. ;

Nemenoff, Raphael A. ;

van Dyk, Linda F. ;

Clambey, Eric T. .

JOURNAL OF IMMUNOLOGY, 2018, 200 (01) :3-22

[8] Stabilizing Antibody Cocktails for Mass Cytometry [J].

Schulz, Axel R. ;

Baumgart, Sabine ;

Schulze, Julia ;

Urbicht, Marie ;

Gruetzkau, Andreas ;

Mei, Henrik E. .

CYTOMETRY PART A, 2019, 95A (08) :910-916

[9] High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples [J].

Thrash, Emily M. ;

Kleinsteuber, Katja ;

Hathaway, Emma S. ;

Nazzaro, Matthew ;

Haas, Eric ;

Hodi, F. Stephen ;

Severgnini, Mariano .

STAR PROTOCOLS, 2020, 1 (02)

[10] diffcyt: Differential discovery in high-dimensional cytometry via high-resolution clustering [J].

Weber, Lukas M. ;

Nowicka, Malgorzata ;

Soneson, Charlotte ;

Robinson, Mark D. .

COMMUNICATIONS BIOLOGY, 2019, 2 (1)

← 1 2 →