Anti-cancer effect of nano-encapsulated boswellic acids, curcumin and naringenin against HepG-2 cell line

Backgroundliver cancer is one of the most common cancers in the world. So far, there is no gold standard treatment for hepatocellular carcinoma. We conducted this in vitro study to assess the effect of three natural products: Boswellic acids, curcumin and naringin versus corresponding nanoparticles (NPs) on Hep G2 cells proliferation.MethodsBoswellic acid, curcumin, naringin-loaded NPs were prepared using nanoprecipitation method. Human liver (HepG2) cell line was cultured in Dulbecco's modified Eagle's medium (DMEM). The cell growth inhibition and cytotoxicity were evaluated by MTT assay.ResultsBoswellic acid, curcumin, naringin were able to inhibit HepG2 cells proliferation. IC50 at 24 h, 48 h showed significant lower values in NPs versus Free herbs. IC50 values of free Boswellic acids and NPs at 24 h were (24.60 & PLUSMN; 1.89 and 7.78 & PLUSMN; 0.54, P < 0.001), at 48 h were (22.45 & PLUSMN; 1.13 and 5.58 & PLUSMN; 0.27, P < 0.001) respectively. IC50 values of free curcumin and NPs at 24 h were (5.89 & PLUSMN; 0.8 and 3.46 & PLUSMN; 0.23, P < 0.05), at 48 h were (5.57 & PLUSMN; 0.94 and 2.51 & PLUSMN; 0.11, P < 0.05), respectively. For free and naringenin NPs, IC50 values at 24 h were (14.57 & PLUSMN; 1.78 and 7.25 & PLUSMN; 0.17, P < 0.01), at 48 h were (11.37 & PLUSMN; 1.45 and 5.21 & PLUSMN; 0.18, P < 0.01) respectively.ConclusionBoswellic acid, curcumin, naringin and their nanoprecipitation prepared nanoparticles suppressed Hep G2 cells proliferation.