Simultaneous determination of six vitamin A substances by multi-wavelength HPLC and application to different marine shellfishes

被引：5

作者：

Xing, Long ^{[1
]}

Gao, Qi ^{[1
]}

Huang, Xinyi ^{[1
]}

Xue, Yuhan ^{[1
]}

Ding, Leshan ^{[1
]}

Li, Jing ^{[1
]}

Hou, Hu ^{[1
]}

Wang, Jingfeng ^{[1
]}

Dong, Ping ^{[1
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

来源：

JOURNAL OF FOOD COMPOSITION AND ANALYSIS | 2023年 / 115卷

基金：

国家重点研发计划;

关键词：

Vitamin A substances; HPLC-DAD; Marine shellfish; Ultrasound-assisted extraction; FAT-SOLUBLE VITAMINS; BIOCHEMICAL-COMPOSITION; BETA-CAROTENE; RETINOL; MILK; TOCOPHEROLS; PALMITATE; CHEMISTRY; BENEFITS;

D O I：

10.1016/j.jfca.2022.105039

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

An HPLC-DAD method was developed for the simultaneous analytical determination of six vitamin A substances (retinol, retinyl acetate, retinyl palmitate, retinoic acid, retinal and 0-carotene) and applied to the analysis of vitamin A substances in five marine shellfishes. The chromatographic column was a YMC-0 carotene C30 column (250 x 4.6 mm, 3 mu m), and the mobile phases were selected as A (methanol), B (ultrapure water) and C (methyl tert-butyl ether). The initial ratio of the mobile phase was A:B:C= 90:1:9, which was maintained for 8 min, while the ratio of the three was changed to A:B:C= 35:0:65 and continued for 8 min, and finally returned to the initial ratio within 0.1 min. Ultrasound-assisted direct extraction was investigated, and the optimal extraction condi-tions were determined by exploring different sample-to-liquid ratios, extractants, extraction times and extraction temperatures. The method was validated and the established assay showed good linearity in the range of 0.5-50 mu g/mL with good precision and accuracy. The experimental results showed that retinol, retinyl palmitate and 0-carotene were present in the shellfish and there were seasonal variations of vitamin A substances in different marine economic shellfish.

引用

页数：7