Application of Grafting Method in Resistance Identification of Sweet Potato Virus Disease and Resistance Evaluation of Elite Sweet Potato [Ipomoea batatas (L.) Lam] Varieties

被引：4

作者：

Huang, Hong ^{[1
,2
]}

Han, Haohao ^{[1
,2
]}

Lei, Yayun ^{[1
,2
]}

Qiao, Huanhuan ^{[1
,2
]}

Tang, Daobin ^{[1
,2
,3
]}

Han, Yonghui ^{[1
,2
]}

Deng, Zhenpeng ^{[1
,2
]}

Mao, Limin ^{[1
,2
]}

Wu, Xuli ^{[1
,2
]}

Zhang, Kai ^{[1
,2
,3
]}

Wang, Jichun ^{[1
,2
,3
]}

Lv, Changwen ^{[1
,2
,3
]}

机构：

[1] Southwest Univ, Coll Agron & Biotechnol, Chongqing 400715, Peoples R China

[2] Key Lab Biol & Genet Breeding Tuber & Root Crops C, Chongqing 400715, Peoples R China

[3] Southwest Univ, State Cultivat Base Crop Stress Biol Southern Mt L, Chongqing 400715, Peoples R China

来源：

PLANTS-BASEL | 2023年 / 12卷 / 04期

关键词：

grafting; SPVD; symptomatic detection; NCM-ELISA; QRT-PCR; FEATHERY-MOTTLE-VIRUS; POTYVIRUS; YIELD; SPVD;

D O I：

10.3390/plants12040957

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Sweet potato virus disease (SPVD) is one of the main virus diseases in sweet potato [Ipomoea batatas (L.) Lam] that seriously affects the yield of sweet potato. Therefore, the establishment of a simple, rapid and effective method to detect SPVD is of great significance for the early warning and prevention of this disease. In this study, the experiment was carried out in two years to compare the grafting method and side grafting method for three sweet potato varieties, and the optimal grafting method was selected. After grafting with seedlings infected with SPVD, the symptomatic diagnosis and serological detection were performed in 86 host varieties, and the differences in SPVD resistance were determined by fluorescence quantitative PCR (qRT-PCR) and nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). The results showed that the survival rate of grafting by insertion method was significantly higher than that by side grafting method, and the disease resistance of different varieties to sweet potato virus disease was tested. The detection method established in this study can provide theoretical basis for identification and screening of resistant sweet potato varieties.

引用

页数：15

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