A Rapid Immunochromatographic Method Based on Gold Nanoparticles for the Determination of Imidacloprid on Fruits and Vegetables

被引：11

作者：

Suryoprabowo, Steven ^{[1
]}

Wu, Aihong ^{[1
]}

Liu, Liqiang ^{[1
]}

Kuang, Hua ^{[1
]}

Xu, Chuanlai ^{[1
]}

Guo, Lingling ^{[1
]}

机构：

[1] Jiangnan Univ, Sch Food Sci & Technol, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

来源：

FOODS | 2023年 / 12卷 / 03期

关键词：

gold immunochromatography test; imidacloprid; monoclonal antibody; strip test; LINKED-IMMUNOSORBENT-ASSAY; LIQUID-CHROMATOGRAPHY; IMMUNOASSAY; PESTICIDES; THIAMETHOXAM; HONEY;

D O I：

10.3390/foods12030512

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Imidacloprid (IMP) is toxic and a potential carcinogen that is most widely used as an insecticide for pest control and seed treatment. It is important to produce a rapid and sensitive assay for on-site monitoring. We have developed a novel lateral flow assay (LFA) using a sensitive monoclonal antibody (mAb) for monitoring IMP residues on fruits and vegetables. The 50% inhibition concentration result that was found when using the ELISA method was 0.247 ng mL(-1), with the cut-off limits using the LFA method the result was 10 ng mL(-1) (0.01 M PBS), and in the samples it was 20 ng mL(-1) (with a recovery rate of 96-104.7% for Chinese cabbage, cowpea, apple, and pear samples, respectively). All of the results can be determined within seven minutes. The proposed LFA method is a valid, quick, and stable assay for the on-site detection of IMP in large numbers of samples.

引用

页数：9

共 34 条

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