Action of BjussuMP-II, a snake venom metalloproteinase isolated from Bothrops jararacussu venom, on human neutrophils

被引：9

作者：

Lisita, K. ^{[1
,2
]}

Silva, M. D. S. ^{[1
]}

Santana, H. M. ^{[1
]}

Ikenohuchi, Y. J. ^{[1
]}

Paloschi, M. V. ^{[1
]}

Rego, C. M. A. ^{[1
]}

Serrath, S. N. ^{[1
]}

Lima, A. M. ^{[3
,4
]}

Sousa, M. N. ^{[3
,4
]}

Soares, A. M. ^{[4
]}

Setubal, S. S. ^{[1
]}

Zuliani, J. P. ^{[1
,5
]}

机构：

[1] Fundacao Oswaldo Cruz, FIOCRUZ Rondonia, Lab Imunol Celular Aplicada Saude, Porto Velho, RO, Brazil

[2] Univ Fed Acre, Rio Branco, AC, Brazil

[3] INCT EPIAMO, Inst Nacl Ciencia Tecnol Epidemiol Amazonia, Porto Velho, RO, Brazil

[4] Fundacao Oswaldo Cruz, FIOCRUZ Rondonia, LABIOPROT, Lab Biotecnol Prot Compo Bioat Aplicados Saude, Porto Velho, RO, Brazil

[5] Univ Fed Rondonia, Dept Med, UNIR, Porto Velho, RO, Brazil

来源：

TOXICON | 2023年 / 222卷

关键词：

Snake venom; metalloproteinase; neutrophils; mediators; AMINO-ACID OXIDASE; LEUKOCYTE RECRUITMENT; ADHESION MOLECULES; ASPER; MECHANISMS; RELEASE;

D O I：

10.1016/j.toxicon.2022.106992

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Snake venom metalloproteinases (SVMPs) are enzymatic proteins present in large amounts in snake venoms presenting proteolytic, hemorrhagic, and coagulant activities. BjussuMP-II, a class P-I SVMP, isolated from the Bothrops jararacussu snake venom does not have relevant hemorrhagic activity but presents fibrinolytic, fibrinogenolytic, antiplatelet, gelatinolytic, and collagenolytic action. This study aimed to verify the action of BjussuMP-II on human neutrophil functionality focusing on the lipid bodies formation and hydrogen peroxide production, the release of dsDNA through colorimetric and microscopic assays, and cytokines by immunoenzymatic assays. Results showed that BjussuMP-II at concentrations of 1.5 up to 50 mu g/mL for 24 h is not toxic to human neutrophils using an MTT assay. Under non-cytotoxic concentrations, BjussuMP-II can induce an increase in the formation of lipid bodies, production of hydrogen peroxide and cytokines [tumor necrosis factor- a (TNF-a), interleukin-1 ss (IL-1 ss), interleukin-6 (IL-6) and interleukin-8 (IL-8)] liberation and, the release of dsDNA to form NETs. Taken together, the data obtained show for the first time that BjussuMP-II has a pro-inflammatory action and activates human neutrophils that can contribute to local damage observed in snakebite victims.

引用

页数：8

共 40 条

[11] Activation of J77A.1 Macrophages by Three Phospholipases A2 Isolated from Bothrops atrox Snake Venom [J].

Furtado, Juliana L. ;

Oliveira, George A. ;

Pontes, Adriana S. ;

Setubal, Sulamita da S. ;

Xavier, Caroline V. ;

Lacouth-Silva, Fabianne ;

Lima, Beatriz F. ;

Zaqueo, Kayena D. ;

Kayano, Anderson M. ;

Calderon, Leonardo A. ;

Stabeli, Rodrigo G. ;

Soares, Andreimar M. ;

Zuliani, Juliana P. .

BIOMED RESEARCH INTERNATIONAL, 2014, 2014

[12] Snake venom metalloproteinases:: Their role in the pathogenesis of local tissue damage [J].

Gutiérrez, JM ;

Rucavado, A .

BIOCHIMIE, 2000, 82 (9-10) :841-850

[13] Hemorrhage Caused by Snake Venom Metalloproteinases: A Journey of Discovery and Understanding [J].

Gutierrez, Jose Maria ;

Escalante, Teresa ;

Rucavado, Alexandra ;

Herrera, Cristina .

TOXINS, 2016, 8 (04)

[14] NETosis and lack of DNase activity are key factors in Echis carinatus venom-induced tissue destruction [J].

Katkar, Gajanan D. ;

Sundaram, Mahalingam S. ;

NaveenKumar, Somanathapura K. ;

Swethakumar, Basavarajaiah ;

Sharma, Rachana D. ;

Paul, Manoj ;

Vishalakshi, Gopalapura J. ;

Devaraja, Sannaningaiah ;

Girish, Kesturu S. ;

Kemparaju, Kempaiah .

NATURE COMMUNICATIONS, 2016, 7

[15] Molecular and functional characterization of a new non-hemorrhagic metalloprotease from Bothrops jararacussu snake venom with antiplatelet activity [J].

Marcussi, Silvana ;

BernardeS, Carolina P. ;

Santos-Filho, Norival A. ;

Mazzi, Mauricio V. ;

Oliveira, Clayton Z. ;

Izidoro, Luiz Fernando M. ;

Fuly, Andre L. ;

Magro, Angelo J. ;

Braz, Antonio S. K. ;

Fontes, Marcos R. M. ;

Giglio, Jose R. ;

Soares, Andreimar M. .

PEPTIDES, 2007, 28 (12) :2328-2339

[16] Snake venom metalloproteinases [J].

Markland, Francis S., Jr. ;

Swenson, Stephen .

TOXICON, 2013, 62 :3-18

[17] Immunomodulatory actions and epigenetic alterations induced by proteases from Bothrops snake venoms in human immune cells [J].

Menaldo, Danilo L. ;

Costa, Tassia R. ;

Ribeiro, Diego L. ;

Zambuzi, Fabiana A. ;

Antunes, Lusania M. G. ;

Castro, Fabiola A. ;

Frantz, Fabiani G. ;

Sampaio, Suely V. .

TOXICOLOGY IN VITRO, 2019, 61

[18] Snake Venom Metalloproteinases (SVMPs): A structure-function update [J].

Olaoba, Olamide Tosin ;

dos Santos, Patty Karina ;

Selistre-de-Araujo, Heloisa Sobreiro ;

de Souza, Dulce Helena Ferreira .

TOXICON-X, 2020, 7

[19] Dynamics and functions of lipid droplets [J].

Olzmann, James A. ;

Carvalho, Pedro .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2019, 20 (03) :137-155

[20] Cytosolic phospholipase A2-α participates in lipid body formation and-PGE2 release in human neutrophils stimulated with an l-amino acid oxidase from Calloselasma rhodostoma venom [J].

Paloschi, Mauro Valentino ;

Lopes, Jessica Amaral ;

Boeno, Charles Nunes ;

Souza Silva, Milena Daniela ;

Evangelista, Jaina Rodrigues ;

Pontes, Adriana Silva ;

Setubal, Sulamita da Silva ;

Alves Rego, Cristina Matiele ;

Nery, Neriane Monteiro ;

Ferreira e Ferreira, Alex Augusto ;

Pires, Weverson Luciano ;

Felipin, Katia Paula ;

Melim Ferreira, Gabriel Eduardo ;

Bozza, Patricia Torres ;

Zuliani, Juliana Pavan .

SCIENTIFIC REPORTS, 2020, 10 (01)

← 1 2 3 4 →