Antioxidant, antimetastatic and apoptotic potential of silver nanoparticles synthesized by leaf and callus extracts of Hyptis suaveolens L.

Green synthesis of silver nanoparticles using plant extracts as reducing agents is an environmental friendly and cost-effective approach. This study aims to provide a detailed overview on the antioxidant, antimetastatic and apoptotic potential of leaf derived (L-AgNPs) and callus derived (C-AgNPs) silver nanoparticles of Hyptis suaveolens. The IC50 value of L-AgNPs was found to be 74.22 mg/ml and for C-AgNPs it was 74.66 mg/ ml. Antimetastatic potential of both L-AgNPs and C-AgNPs were tested on MDA-MB-231 cell line. Both IC50 concentrations of L-AgNPs (74.22 mg/ml) and maximum concentration of 100 mg/ml was used for all the tests conducted. Similar procedure was adopted for C-AgNPs as well. The maximum adhesion inhibition was 75.64% while cellular invasion reduction was 72.66% and wound recovery was 49.61% with respect to LAgNPs. C-AgNPs also showed similar type of results as L-AgNPs when compared to control. The antioxidant potential of L-AgNPs and C-AgNPs was tested by using DPPH radical scavenging activity, FRAP activity and reducing power. The L-AgNPs and C-AgNPs showed highest antioxidant potential when compared to control.& COPY; 2023 SAAB. Published by Elsevier B.V. All rights reserved.