The Circulating miRNA Profile of Chronic Hepatitis D and B Patients Is Comparable but Differs from That of Individuals with HBeAg-Negative HBV Infection

被引：3

作者：

Cavallone, Daniela ^{[1
,2
,3
]}

Ornos, Eric David B. ^{[1
,2
,4
,5
]}

Ricco, Gabriele ^{[1
,2
]}

Oliveri, Filippo ^{[1
,2
]}

Coco, Barbara ^{[1
,2
]}

Colombatto, Piero ^{[1
,2
]}

De Rosa, Laura ^{[6
,7
]}

Dalmacio, Leslie Michelle M. ^{[8
]}

Bonino, Ferruccio ^{[1
,2
,3
]}

Brunetto, Maurizia Rossana ^{[1
,2
,3
,9
]}

机构：

[1] Univ Hosp Pisa, Reference Ctr Tuscany Reg Chron Liver Dis & Canc, Hepatol Unit, Via Paradisa 2, I-56124 Pisa, Italy

[2] Univ Hosp Pisa, Lab Mol Genet & Pathol Hepatitis Viruses, Reference Centreof Tuscany Reg Chron Liver Dis & C, Via Paradisa 2, I-56124 Pisa, Italy

[3] CNR, Inst Biostruct & Bioimaging, Via Am 95, I-80145 Naples, Italy

[4] Univ Philippines Manila, Coll Med, Dept Med Microbiol, Pedro Gil St, Manila 1000, Philippines

[5] Fdn Italiana Fegato, I-34149 Trieste, Italy

[6] CNR, Inst Clin Physiol, I-56124 Pisa, Italy

[7] Univ Trento, Dept Informat Engn & Comp Sci, I-38123 Trento, Italy

[8] Univ Philippines Manila, Coll Med, Dept Biochem & Mol Biol, Pedro Gil St, I-1000 Manila, Philippines

[9] Univ Pisa, Dept Clin & Expt Med, I-56126 Pisa, Italy

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 11期

关键词：

MiR-B-Index; Hepatitis D Virus; Hepatitis B Virus; HDV-RNA; HBV-DNA; DELTA VIRUS-RNA; HEPATOCELLULAR-CARCINOMA; CELL-PROLIFERATION; SURFACE-ANTIGEN; EXPRESSION; REPLICATION; DISEASE; GENOME;

D O I：

10.3390/v15112257

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

miRNAs circulating in whole serum and HBsAg-particles are differentially expressed in chronic hepatitis B (CHB) and HBeAg-negative-HBV infection (ENI); their profiles are unknown in chronic hepatitis D (CHD). Serum- and HBsAg-associated miRNAs were analyzed in 75 subjects of 3 well-characterized groups (CHB 25, CHD 25, ENI 25) using next-generation sequencing (NGS). Overall miRNA profiles were consonant in serum and HBsAg-particles but significantly different according to the presence of hepatitis independently of Hepatitis D Virus (HDV)-co-infection. Stringent (Bonferroni Correction < 0.001) differential expression analysis showed 39 miRNAs upregulated in CHB vs. ENI and 31 of them also in CHD vs. ENI. miRNA profiles were coincident in CHB and CHD with only miR-200a-3p upregulated in CHB. Three miRNAs (miR-625-3p, miR-142-5p, and miR-223-3p) involved in immune response were upregulated in ENI. All 3 hepatocellular miRNAs of MiR-B-Index (miR-122-5p, miR-99a-5p, miR-192-5p) were overexpressed in both CHB and CHD patients. In conclusion, CHD and CHB patients showed highly similar serum miRNA profiling that was significantly different from that of individuals with HBeAg-negative infection and without liver disease.

引用

页数：16

共 62 条

[1] Circulating microRNA-122 levels are important predictor of hepatitis B virus surface antigen seroclearance [J].