Melatonin attenuates lipopolysaccharide-induced immune dysfunction in dendritic cells

被引:6
作者
Qin, Tao [1 ,2 ,3 ]
Feng, Danni [1 ]
Zhou, Bangyue [4 ]
Bai, Lirong [4 ]
Zhou, Shengjie [5 ]
Du, Jiangtao [6 ]
Xu, Gang [5 ]
Yin, Yinyan [4 ,7 ]
机构
[1] Yangzhou Univ, Coll Vet Med, Yangzhou, Jiangsu, Peoples R China
[2] Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou, Jiangsu, Peoples R China
[3] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Jiangsu, Peoples R China
[4] Yangzhou Univ, Coll Med, Yangzhou, Jiangsu, Peoples R China
[5] Yangzhou Univ, Northern Jiangsu Peoples Hosp, Clin Med Coll, Dept Burn & Plast Surg, Yangzhou, Jiangsu, Peoples R China
[6] Hangzhou Med Coll, Lab Anim Ctr, Hangzhou, Zhejiang, Peoples R China
[7] Yangzhou Univ, Guangling Coll, Yangzhou, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Melatonin; Dendritic cells; Immunological stress; Acute lung injury; ACUTE LUNG INJURY; INDUCED PULMONARY-FIBROSIS; ELASTASE INHIBITOR; ANIMAL-MODELS; TNF-ALPHA; RECEPTORS; PATHWAY; MATURATION; CROSSTALK; RESPONSES;
D O I
10.1016/j.intimp.2023.110282
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Melatonin, a ubiquitous hormone, is principally secreted from pineal gland in mammals and possesses strong antioxidant and anti-inflammatory properties. However, its specific roles in the immune functions of dendritic cells (DCs) during acute lung injury (ALI) remain unknown. In this study, we found that melatonin restored the body weight, decreased the lung weight/body weight ratio, alleviated the histopathological lung injury, and decreased the levels of cytokines (tumor necrosis factor-& alpha; (TNF-& alpha;), interleukin (IL)-12p70, IL-17, and IL-10) in bronchoalveolar lavage fluid of the lipopolysaccharide (LPS)-induced ALI murine model. Moreover, melatonin inhibited the major histocompatibility complex II (MHCII) expression of lung CD11b+ DCs after LPS challenge in vivo. In vitro, melatonin reversed the shape index, promoted the endocytosis, and inhibited phenotypic expression of MHCII, CD40, CD80, and CD86 in LPS-activated DCs. Furthermore, melatonin decreased the expression of an activated marker, CD69, and the secretion of pro-inflammatory cytokines (TNF-& alpha;, IL-12p70, and IL-17) after LPS challenge. It hampered the LPS-activated DCs migration by downregulating the C-C chemokine receptor 7 (CCR7) expression, and then weakened the ability of LPS-induced DCs to stimulate allogeneic CD4+ T cell proliferation. Melatonin shaped the immune function of DCs in a nuclear factor erythroid-2-related factor 2 (Nrf-2)/heme oxygenase-1 (HO-1) axis-dependent manner. These findings indicate that melatonin protects DCs from ALI-induced immunological stress and may be used to develop novel DC-targeting strategies for ALI therapy.
引用
收藏
页数:12
相关论文
共 46 条
[11]   Acute Lung Injury: IL-17A-Mediated Inflammatory Pathway and Its Regulation by Curcumin [J].
Gouda, Mahesh Manjunath ;
Bhandary, Yashodhar Prabhakar .
INFLAMMATION, 2019, 42 (04) :1160-1169
[12]   Nrf2-A Molecular Target for Sepsis Patients in Critical Care [J].
Gunne, Sandra ;
Heinicke, Ulrike ;
Parnham, Michael J. ;
Laux, Volker ;
Zacharowski, Kai ;
von Knethen, Andreas .
BIOMOLECULES, 2020, 10 (12) :1-22
[13]   Melatonin-A pleiotropic, orchestrating regulator molecule [J].
Hardeland, Ruediger ;
Cardinali, Daniel P. ;
Srinivasan, Venkatramanujam ;
Spence, D. Warren ;
Brown, Gregory M. ;
Pandi-Perumal, Seithikurippu R. .
PROGRESS IN NEUROBIOLOGY, 2011, 93 (03) :350-384
[14]   Crosstalk between T Lymphocytes and Dendritic Cells [J].
Hivroz, Claire ;
Chemin, Karine ;
Tourret, Marie ;
Bohineust, Armelle .
CRITICAL REVIEWS IN IMMUNOLOGY, 2012, 32 (02) :139-155
[15]   Genetic variation of melatonin productivity in laboratory mice under domesticationd [J].
Kasahara, Takaoki ;
Abe, Kuniya ;
Mekada, Kazuyuki ;
Yoshiki, Atsushi ;
Kato, Tadafumi .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (14) :6412-6417
[16]   IL-17 and Th17 Cells [J].
Korn, Thomas ;
Bettelli, Estelle ;
Oukka, Mohamed ;
Kuchroo, Vijay K. .
ANNUAL REVIEW OF IMMUNOLOGY, 2009, 27 :485-517
[17]   Importance of myeloid dendritic cells in persistent airway disease after repeated allergen exposure [J].
Koya, T ;
Kodama, T ;
Takeda, K ;
Miyahara, N ;
Yang, ES ;
Taube, C ;
Joetham, A ;
Park, JW ;
Dakhama, A ;
Gelfand, EW .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2006, 173 (01) :42-55
[18]   Regulatory T Cells and Acute Lung injury: Cytokines, Uncontrolled inflammation, and Therapeutic implications [J].
Lin, Shihui ;
Wu, Hua ;
Wang, Chuanjiang ;
Xiao, Zhibo ;
Xu, Fang .
FRONTIERS IN IMMUNOLOGY, 2018, 9
[19]   Losartan inhibits conventional dendritic cell maturation and Th1 and Th17 polarization responses: Novel mechanisms of preventive effects on lipopolysaccharide-induced acute lung injury [J].
Liu, Jun ;
Zhang, Peng-Shu ;
Yu, Qing ;
Liu, Ling ;
Yang, Yi ;
Guo, Feng-Mei ;
Qiu, Hai-Bo .
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 2012, 29 (02) :269-276
[20]   Mesenchymal stem cells induce dendritic cell immune tolerance via paracrine hepatocyte growth factor to alleviate acute lung injury [J].
Lu, Zhonghua ;
Chang, Wei ;
Meng, Shanshan ;
Xu, Xiuping ;
Xie, Jianfeng ;
Guo, Fengmei ;
Yang, Yi ;
Qiu, Haibo ;
Liu, Ling .
STEM CELL RESEARCH & THERAPY, 2019, 10 (01)