Molecular characterization of a novel victorivirus (order Ghabrivirales, family Totiviridae) infecting Metarhizium anisopliae

被引：1

作者：

Camargo, Matheus da Silva ^{[1
,2
]}

Geremia, Felipe ^{[1
,2
]}

Sbaraini, Nicolau ^{[3
]}

Staats, Charley Christian ^{[1
,2
]}

Silva Filho, Marcio ^{[4
]}

Schrank, Augusto ^{[1
,2
,5
]}

机构：

[1] Univ Fed Rio Grande do Sul, Biotechnol Ctr, Porto Alegre, Brazil

[2] Univ Fed Rio Grande do Sul, Deparment Mol Biol & Biotechnol, Porto Alegre, Brazil

[3] Univ Western Australia, Sch Mol Sci, Perth, WA 6009, Australia

[4] Univ Sao Paulo, Dept Genet, ESALQ, Piracicaba, Brazil

[5] Univ Fed Rio Grande do Sul, Biotechnol Ctr, Cellular & Mol Biol Filamentous Fungi Lab, 9500 Bento Goncalves AveLab 217,Campus Box 43421, BR-91501970 Porto Alegre, RS, Brazil

来源：

ARCHIVES OF VIROLOGY | 2023年 / 168卷 / 03期

基金：

巴西圣保罗研究基金会;

关键词：

FUNGUS;

D O I：

10.1007/s00705-023-05716-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Here, we report the occurrence and complete genome sequence of a novel victorivirus infecting Metarhizium anisopliae, named "Metarhizium anisopliae victorivirus 1" (MaVV1). The genome is 5353 bp in length and contains two open reading frames (ORFs), encoding a coat protein and an RNA-dependent RNA polymerase (RdRp), that overlap at the octanucleotide sequence AUGAGUAA. These ORFs showed sequence similarity to the corresponding ORFs of Ustilaginoidea virens RNA virus L (68.23%) and Ustilaginoidea virens RNA virus 13 (58.11%), respectively, both of which belong to the family Totiviridae. Phylogenetic analysis based on RdRp sequences revealed that MaVV1 clustered with members of the genus Victorivirus. This is the first genome sequence reported for a virus belonging to the genus Victorivirus infecting the entomopathogenic fungus M. anisopliae.

引用

页数：4

共 23 条

[1] ISOLATION OF VIRAL DOUBLE-STRANDED RNAS USING A LICL FRACTIONATION PROCEDURE [J].

DIAZRUIZ, JR ;

KAPER, JM .

PREPARATIVE BIOCHEMISTRY, 1978, 8 (01) :1-17

[2]

Evans G A., Cell, V61, P17, DOI DOI 10.1016/0092-8674(90)90210-6

[3] The Polymycovirus-Mediated Growth Enhancement of the Entomopathogenic Fungus Beauveria bassiana Is Dependent on Carbon and Nitrogen Metabolism [J].

Filippou, Charalampos ;

Diss, Rebecca M. ;

Daudu, John O. ;

Coutts, Robert H. A. ;

Kotta-Loizou, Ioly .

FRONTIERS IN MICROBIOLOGY, 2021, 12

[4] Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana [J].

Filippou, Charalampos ;

Garrido-Jurado, Inmaculada ;

Meyling, Nicolai V. ;

Quesada-Moraga, Enrique ;

Coutts, Robert H. A. ;

Kotta-Loizou, Ioly .

VIRUSES-BASEL, 2018, 10 (12)

[5] Characterization of mycoviruses and analyses of chitinase secretion in the biocontrol fungus Metarhizium anisopliae [J].

Giménez-Pecci, MD ;

Bogo, MR ;

Santi, L ;

de Moraes, CK ;

Corrêa, CT ;

Vainstein, MH ;

Schrank, A .

CURRENT MICROBIOLOGY, 2002, 45 (05) :334-339

[6] New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0 [J].

Guindon, Stephane ;

Dufayard, Jean-Francois ;

Lefort, Vincent ;

Anisimova, Maria ;

Hordijk, Wim ;

Gascuel, Olivier .

SYSTEMATIC BIOLOGY, 2010, 59 (03) :307-321

[7] Monitoring of the field application of Metarhizium anisopliae in Brazil revealed high molecular diversity of Metarhizium spp in insects, soil and sugarcane roots [J].

Iwanicki, Natasha Sant'Anna ;

Pereira, Alessandro Alves ;

Zanardo Botelho, Ana Beatriz Riguetti ;

Rezende, Janayne Maria ;

Moral, Rafael de Andrade ;

Zucchi, Maria Imaculada ;

Delalibera Junior, Italo .

SCIENTIFIC REPORTS, 2019, 9 (1)

[8]

Jnior D., 2017, Plant Gene, V11, P49, DOI [10.1016/j.plgene.2017.01.001, DOI 10.1016/J.PLGENE.2017.01.001]

[9] MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform [J].

Katoh, K ;

Misawa, K ;

Kuma, K ;

Miyata, T .

NUCLEIC ACIDS RESEARCH, 2002, 30 (14) :3059-3066

[10] A Novel Totivirus Naturally Occurring in Two Different Fungal Genera [J].

Khalifa, Mahmoud E. ;

MacDiarmid, Robin M. .

FRONTIERS IN MICROBIOLOGY, 2019, 10

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