An aggregation of human embryonic and trophoblast stem cells reveals the role of trophectoderm on epiblast differentiation

被引：0

作者：

Wu, Xulun ^{[1
,2
,3
,4
]}

Zhao, Wentao ^{[1
,2
,3
,4
]}

Wu, Hao ^{[1
,2
,3
]}

Zhang, Qiancheng ^{[1
,2
,3
,4
]}

Wang, Yiming ^{[1
,2
,3
,4
]}

Yu, Kunyuan ^{[1
,2
,3
,4
]}

Zhai, Jinglei ^{[1
,2
,3
]}

Mo, Fan ^{[1
,2
,3
,4
]}

Wang, Meijiao ^{[1
,2
,3
]}

Li, Shiwen ^{[1
,2
,3
]}

Zhu, Xili ^{[1
,2
,3
]}

Liang, Xiaoyan ^{[5
]}

Hu, Baoyang ^{[1
,2
,3
,4
]}

Liu, Guang-Hui ^{[2
,3
,4
,6
]}

Wu, Jun ^{[7
,8
,9
]}

Wang, Hongmei ^{[1
,2
,3
,4
]}

Guo, Fan ^{[1
,2
,3
,4
]}

Yu, Leqian ^{[1
,2
,3
,4
]}

机构：

[1] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing, Peoples R China

[2] Chinese Acad Sci, Inst Stem Cell & Regenerat, Beijing, Peoples R China

[3] Beijing Inst Stem Cell & Regenerat Med, Beijing, Peoples R China

[4] Univ Chinese Acad Sci, Beijing, Peoples R China

[5] Sun Yat Sen Univ, Affiliated Hosp 6, Reprod Med Res Ctr, Guangzhou, Peoples R China

[6] Chinese Acad Sci, Inst Zool, State Key Lab Membrane Biol, Beijing, Peoples R China

[7] Univ Texas Southwestern Med Ctr Dallas, Dept Mol Biol, Dallas, TX USA

[8] Univ Texas Southwestern Med Ctr Dallas, Hamon Ctr Regenerat Sci & Med, Dallas, TX USA

[9] Univ Texas Southwestern Med Ctr Dallas, Cecil H & Ida Green Ctr Reprod Biol Sci, Dallas, TX USA

来源：

CELL PROLIFERATION | 2023年 / 56卷 / 05期

基金：

中国国家自然科学基金;

关键词：

SELF-ORGANIZATION; MESODERM;

D O I：

10.1111/cpr.13492

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The interactions between extra-embryonic tissues and embryonic tissues are crucial to ensure proper early embryo development. However, the understanding of the crosstalk between the embryonic tissues and extra-embryonic tissues is lacking, mainly due to ethical restrictions, difficulties in obtaining natural human embryos, and lack of appropriate in vitro models. Here by aggregating human embryonic stem cells (hESCs) with human trophoblast stem cells (hTSCs), we revealed the hESCs robustly self-organized into a unique asymmetric structure which the primitive streak (PS) like cells exclusively distributed at the distal end to the TS-compartment, and morphologically flattened cells, presumed to be the extra-embryonic mesoderm cells (EXMC) like cells, were induced at the proximal end to hTSCs. Our study revealed two potential roles of extra-embryonic trophectoderm in regulating the proper PS formation during gastrulation and EXMCs induction from the human epiblast.

引用

页数：12

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