New Data on the Features of Skin Barrier in Hidradenitis Suppurativa

被引:3
作者
Somogyi, Orsolya [1 ,2 ,3 ]
Dajnoki, Zsolt [1 ,2 ]
Szabo, Lilla [1 ,2 ,3 ]
Gaspar, Krisztian [1 ,2 ]
Hendrik, Zoltan [4 ]
Zouboulis, Christos C. [5 ,6 ]
Docs, Klaudia [7 ]
Szucs, Peter [7 ]
Dull, Katalin [1 ]
Torocsik, Daniel [1 ,2 ]
Kapitany, Aniko [1 ,2 ]
Szegedi, Andrea [1 ,2 ]
机构
[1] Univ Debrecen, Fac Med, MTA Ctr Excellence, Dept Dermatol, H-4032 Debrecen, Hungary
[2] ELKH DE Allergol Res Grp, H-4032 Debrecen, Hungary
[3] Univ Debrecen, Petrany Gyula Doctoral Sch, H-4032 Debrecen, Hungary
[4] Univ Debrecen, Fac Med, Dept Pathol, H-4032 Debrecen, Hungary
[5] Brandenburg Med Sch Theodor Fontane, Dessau Med Ctr, Dept Dermatol Venereol Allergol & Immunol, D-06847 Dessau, Germany
[6] Fac Hlth Sci Brandenburg, D-06847 Dessau, Germany
[7] Univ Debrecen, Fac Med, Dept Anat Histol & Embryol, H-4032 Debrecen, Hungary
关键词
hidradenitis suppurativa; permeability barrier; desmosome; tight junction; keratinocyte; ATOPIC-DERMATITIS; DYSFUNCTION; DISEASE;
D O I
10.3390/biomedicines11010127
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hidradenitis suppurativa (HS) is a Th1/17-driven inflammatory skin disease of the apocrine gland-rich (AGR) skin regions, where keratinocytes seem to be the crucial drivers of the initial pathogenic steps. However, the possible role of permeability barrier alteration in activating keratinocytes during HS development has not been clarified. We compared the major permeability barrier elements of non-lesional HS (HS-NL; n = 10) and lesional HS (HS-L; n = 10) skin with healthy AGR regions (n = 10) via RT-qPCR and immunohistochemistry. Stratum corneum components related to cornified envelope formation, corneocyte desquamation and (corneo)desmosome organization were analyzed along with tight junction molecules and barrier alarmins. The permeability barrier function was also investigated with transepidermal water loss (TEWL) measurements (n = 16). Junction structures were also visualized using confocal microscopy. At the gene level, none of the investigated molecules were significantly altered in HS-NL skin, while 11 molecules changed significantly in HS-L skin versus control. At the protein level, the investigated molecules were similarly expressed in HS-NL and AGR skin. In HS-L skin, only slight changes were detected; however, differences did not show a unidirectional alteration, as KRT1 and KLK5 were detected in decreased levels, and KLK7, KRT6 and DSG1 in increased levels. No significant differences in TEWL or the expression of junction structures were assessed. Our findings suggest that the permeability barrier is not significantly damaged in HS skin and permeability barrier alterations are not the driver factors of keratinocyte activation in this disease.
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页数:12
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