Pontibacter kalidii sp. nov., isolated from rhizosphere soil of Kalidium foliatum

A Gram-negative, aerobic, gliding motile, rod-shaped bacterium, designated XAAS- 72(T), was isolated from the rhizosphere soil of Kalidium foliatum sampled in the Xinjiang Uyghur Autonomous Region, PR China. Cells grew at 4-45 degrees C, pH 5.0-8.0 and 0-8% NaCl, with optimal growth at 20-30 degrees C, pH 6.0-7.0 and 1-2 % NaCl. Strain XAAS- 72(T) is closely related to members of the genus Pontibacter, namely Pontibacter korlensis CCTCC AB 206081T (97.6%) and Pontibacter flavimaris ACCC 19859T (97.2 %), and <94.6 % related to other currently described Pontibacter strains. The average nucleotide identity values between XAAS- 72T and P. korlensis CCTCC AB 206081(T) and P. flavimaris ACCC 19859(T) were 77.9 and 86.9 %, respectively; the corresponding digital DNA-DNA hybridization values were 21.7 and 31.8 %. Menaquinone- 7 was the predominant respiratory menaquinone. The polar lipids consisted of phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified glycolipids and five unidentified lipids. The major cellular fatty acids were summed feature 4 (containing iso- C17: 1 I/anteiso-C17: 1 B), summed feature 3 (containing C-16: 1 omega 7c/C-16:1 omega 6c) and iso-C-15:0. The genome length of strain XAAS- 72(T) was 5 054 860 bp with a genomic DNA G+C content of 54.5 mol%. The phenotypic and genotypic data suggest that strain XAAS- 72(T) represents a novel species of the genus Pontibacter, for which the name Pontibacter kalidii sp. nov. is proposed. The strain is XAAS- 72(T) (CGMCC 16594(T)=KCTC 72095(T)).