Systematic Comparison of Commercial Hydrogels Revealed That a Synergy of Laminin and Strain-Stiffening Promotes Directed Migration of Neural Cells

被引：6

作者：

Millesi, Flavia ^{[1
,2
]}

Mero, Sascha ^{[1
,2
]}

Semmler, Lorenz ^{[1
,2
]}

Rad, Anda ^{[1
,2
]}

Stadlmayr, Sarah ^{[1
,2
]}

Borger, Anton ^{[1
,2
]}

Supper, Paul ^{[1
,2
]}

Haertinger, Maximilian ^{[1
,2
]}

Ploszczanski, Leon ^{[3
]}

Windberger, Ursula ^{[4
]}

Weiss, Tamara ^{[1
,2
]}

Naghilou, Aida ^{[1
,2
,6
]}

Radtke, Christine ^{[1
,2
,5
]}

机构：

[1] Med Univ Vienna, Dept Plast Reconstruct & Aesthet Surg, Res Lab, A-1090 Vienna, Austria

[2] Austrian Cluster Tissue Regenerat, A-1200 Vienna, Austria

[3] Univ Nat Resources & Life Sci, Inst Phys & Mat Sci, A-1190 Vienna, Austria

[4] Med Univ Vienna, Lab Anim Breeding & Husb, Decentralized Biomed Facil, Core Unit, A-1090 Vienna, Austria

[5] Med Univ Vienna, Dept Plast Reconstruct & Aesthet Surg, A-1090 Vienna, Austria

[6] Univ Vienna, Dept Phys Chem, A-1090 Vienna, Austria

来源：

ACS APPLIED MATERIALS & INTERFACES | 2023年 / 15卷 / 10期

基金：

奥地利科学基金会;

关键词：

peripheral nerve regeneration; tissue engineering; biomaterials; directionality; mechanobiology; stiffness; Schwann cells; PERIPHERAL-NERVE REGENERATION; SCHWANN-CELL; TISSUE; PROLIFERATION; BIOMATERIALS; BEHAVIOR; REPAIR; AUTOGRAFTS; TOPOGRAPHY; ELASTICITY;

D O I：

10.1021/acsami.2c20040

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Hydrogels have shown potential in replacing damaged nerve tissue, but the ideal hydrogel is yet to be found. In this study, various commercially available hydrogels were compared. Schwann cells, fibroblasts, and dorsal root ganglia neurons were seeded on the hydrogels, and their morphology, viability, proliferation, and migration were examined. Additionally, detailed analyses of the gels' rheological properties and topography were conducted. Our results demonstrate vast differences on cell elongation and directed migration on the hydrogels. Laminin was identified as the driver behind cell elongation and in combination with a porous, fibrous, and strain-stiffening matrix structure responsible for oriented cell motility. This study improves our understanding of cell-matrix interactions and thereby facilitates tailored fabrication of hydrogels in the future.

引用

页码：12678 / 12695

页数：18

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