The current experimental study aimed to investigate the effects of different nanofluids and heat-light sources on the thermal energy absorption of a laboratory-scale flat plate solar collector (FPSC). Water-based nanofluids containing Al2O3, SiO2, and TiO2 nanoparticles at a concentration of 0.2% were utilized as the service fluid in this study. The thermal behavior of the nanofluids in the FPSC was studied in two stages: heat absorption and heat retention periods. Both the heat absorption and retention periods were conducted for a duration of either 120 or 240 min. Distilled water acted as operating fluid circulating inside the insulated tank and the collector tubing system. Tungsten heat, halogen pencil, infra-red, and mercury vapor lamps were considered as heat-light sources. Among different heat-light sources considered, the collector utilizing a tungsten heat lamp demonstrated the highest thermal efficiency. For this lamp, the efficiencies for 120 min heat absorption were 67.37% for Al2O3, 66.21% for SiO2, 64.64% for TiO2 nanofluids, and 48.78% for base fluid. For the same lamp, during the 240 min heat absorption, these values were 44.72% for Al2O3, 43.42 for SiO2, 42.26% for TiO2 nanofluids, and 37.17% for base fluid. Aside from these, results showed that the thermal efficiency of FPSC with insulation and without insulation was 23% and 20% for 0.2% TiO2 nanofluid, respectively, when halogen pencil lamp was used. Finally, results of considering distilled water or distilled water containing 0.2%, 0.5%, and 1% SDS as service fluid indicated that the highest amount of heat transfer was related to the distilled water. Indeed, heat transfer decreased with increasing SDS content.