Insight on the meat quality and carbonylation profile of breast muscle of broilers in response to chronic heat stress: A proteomic research

被引：6

作者：

Liu, Yingsen ^{[1
]}

Liu, Zhen ^{[1
]}

Xing, Tong ^{[1
]}

Li, Jiaolong ^{[2
]}

Zhang, Lin ^{[1
]}

Jiang, Yun ^{[3
]}

Gao, Feng ^{[1
,4
]}

机构：

[1] Nanjing Agr Univ, Coll Anim Sci & Technol,Key Lab Anim Origin Food P, Jiangsu Collaborat Innovat Ctr Meat Prod & Proc,Qu, Natl Expt Teaching Demonstrat Ctr Anim Sci,Joint I, Nanjing 210095, Peoples R China

[2] Jiangsu Acad Agr Sci, Inst Agroprod Proc, Nanjing 210014, Peoples R China

[3] Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China

[4] Nanjing Agr Univ, Coll Anim Sci & Technol, 1 Weigang, Nanjing 210095, Peoples R China

来源：

FOOD CHEMISTRY | 2023年 / 423卷

基金：

中国国家自然科学基金;

关键词：

Heat stress; Carbonylation; Meat quality; Broiler; PROTEIN CARBONYLATION; ANKYRIN-B; APOPTOSIS; OXIDATION; SPECIFICITY; MECHANISMS; RELEVANCE; INJURY; DOMAIN; SITES;

D O I：

10.1016/j.foodchem.2023.136437

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study was conducted to explore the influences of carbonyl modification on proteins within the breast muscle of heat-stressed broilers and their correlations to decreased meat quality. The results showed that birds that suffered from heat stress had higher lightness, drip loss, shear force value, and hardness, and lower redness and springiness of breast meat than those under normal control and pair fed treatments. Proteomic analysis identified a total of 921 differentially carbonylated sites, which were allocated to 419 proteins. The modified sites included Lys, Pro, Arg, Trp, Cys, His, and Met. Seven motif sequences were detected, where five motifs neighbored Lys and two neighbored Pro. The differentially carbonylated proteins in heat-stressed birds mainly participated in the glycolytic process, collagen fibril organization, calcium homeostasis, and apoptosis. This study provided a unique landscape of the muscular carbonyl modification rule and unraveled the potential impact of carbonylated protein on meat quality.

引用

页数：10

共 40 条

[1] Isoform specificity of ankyrin-B - A site in the divergent C-terminal domain is required for intramolecular association [J].