Arabidopsis hypocotyl growth in darkness requires the phosphorylation of a microtubule-associated protein

被引:0
作者
Arico, Denise Soledad [1 ,2 ,3 ]
Burachik, Natalia B. [1 ]
Wengier, Diego Leonardo [1 ,4 ,5 ]
Mazzella, Maria Agustina [1 ]
机构
[1] Inst Invest Ingn Genet & Biol Mol Hector Torres, RA-2490 Caba, Argentina
[2] Univ Toulouse, Lab Rech Sci Vegetales, CNRS, UPS, F-31320 Auzeville Tolosane, France
[3] UCBL, CNRS, ENS Lyon, Lab Reprod & Dev Plantes,INRAE, Lyon, France
[4] Howard Hughes Med Inst, Shriram Ctr, Dept Chem Engn, 443 Via Ortega, Stanford, CA 94305 USA
[5] Stanford Univ, 443 Via Ortega, Stanford, CA 94305 USA
关键词
Arabidopsis; microtubule; microtubule-associated proteins; skotomophogenesis; phosphoproteome; photoreceptor; PLANT-GROWTH; REGULATORY PROTEIN; CELL ELONGATION; LIGHT; DEGRADATION; HY5; STABILITY; MECHANISM; PLATFORM; MUTANTS;
D O I
10.1111/tpj.16711
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Rapid hypocotyl elongation allows buried seedlings to emerge, where light triggers de-etiolation and inhibits hypocotyl growth mainly by photoreceptors. Phosphorylation/dephosphorylation events regulate many aspects of plant development. Only recently we have begun to uncover the earliest phospho-signaling responders to light. Here, we reported a large-scale phosphoproteomic analysis and identified 20 proteins that changed their phosphorylation pattern following a 20 min light pulse compared to darkness. Microtubule-associated proteins were highly overrepresented in this group. Among them, we studied CIP7 (COP1-INTERACTING-PROTEIN 7), which presented microtubule (MT) localization in contrast to the previous description. An isoform of CIP7 phosphorylated at Serine915 was detected in etiolated seedlings but was undetectable after a light pulse in the presence of photoreceptors, while CIP7 transcript expression decays with long light exposure. The short hypocotyl phenotype and rearrangement of MTs in etiolated cip7 mutants are complemented by CIP7-YFP and the phospho-mimetic CIP7S915D-YFP, but not the phospho-null CIP7S915A-YFP suggesting that the phosphorylated S915CIP7 isoform promotes hypocotyl elongation through MT reorganization in darkness. Our evidence on Serine915 of CIP7 unveils phospho-regulation of MT-based processes during skotomorphogenic hypocotyl growth. In our large-scale phosphoproteome assay to elucidate the initial phospho-signaling components triggered by a short light pulse, we found an enrichment of microtubule (MTs)-associated proteins. Among these proteins, CIP7 (COP1-INTERATING-PROTEIN-7) localizes to MTs and affects hypocotyl growth and MT orientation in dark hypocotyls. Serine915 of CIP7 is dephosphorylated by a brief light pulse. Phosphorylation status of Serine915 regulates this response. Our work establishes a link among skotomorphogenic development, phosphorylation dynamics and MTs-based processes.
引用
收藏
页码:1815 / 1831
页数:17
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