Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles

被引：101

作者：

Davidson, Sean M. ^{[1
]}

Boulanger, Chantal M. ^{[2
]}

Aikawa, Elena ^{[3
]}

Badimon, Lina ^{[4
]}

Barile, Lucio ^{[5
,6
]}

Binder, Christoph J. ^{[7
]}

Brisson, Alain ^{[8
]}

Buzas, Edit ^{[9
]}

Emanueli, Costanza ^{[10
]}

Jansen, Felix ^{[11
]}

Katsur, Miroslava ^{[1
]}

Lacroix, Romaric ^{[12
,13
]}

Lim, Sai Kiang ^{[14
,15
,16
]}

Mackman, Nigel ^{[17
]}

Mayr, Manuel ^{[18
]}

Menasche, Philippe ^{[19
,20
,21
]}

Nieuwland, Rienk ^{[22
,23
]}

Sahoo, Susmita ^{[24
]}

Takov, Kaloyan ^{[18
]}

Thum, Thomas ^{[25
,26
]}

Vader, Pieter ^{[2
,27
]}

Wauben, Marca H. M. ^{[28
]}

Witwer, Kenneth ^{[29
,30
]}

Sluijter, Joost P. G. ^{[20
,21
]}

机构：

[1] UCL, Hatter Cardiovasc Inst, London WC1E 6HX, England

[2] Univ Paris Cite, INSERM, Paris Cardiovasc Res Ctr, Paris, France

[3] Harvard Med Sch, Dept Med, Brigham & Womens Hosp, Ctr Excellence Vasc Biol, Boston, MA 02115 USA

[4] Autonomous Univ Barcelona, IR Hosp Santa Creu & Santa Pau IIBSantPau, CiberCV, Cardiovasc Sci Program ICCC, Barcelona, Spain

[5] Univ Svizzera Italiana, Ente Osped Cantonale, Ist Cardioctr Ticino, Lab Cardiovasc Theranost, CH-6900 Lugano, Switzerland

[6] Univ Svizzera Italiana, Fac Biomed Sci, CH-6900 Lugano, Switzerland

[7] Med Univ Vienna, Dept Lab Med, Vienna, Austria

[8] Univ Bordeaux IPB, CNRS, Mol Imaging & NanoBioTechnol, UMR 5248 CBMN, Bat B14,Allee Geoffroy St Hilaire, F-33600 Pessac, France

[9] Semmelweis Univ, Dept Genet Cell & Immunobiol, HCEMM SU & ELKH SE Immune Proteogen Extracellular, Budapest, Hungary

[10] Imperial Coll London, Natl Heart & Lung Inst, Hammersmith Campus, London W12 0NN, England

[11] Univ Hosp Bonn, Heart Ctr, Dept Internal Med 2, Bonn, Germany

[12] Aix Marseille Univ, Ctr Rech CardioVasc & Nutr C2VN, Inst Natl Rech Agr Alimentat & Environm INRAE, INSERM 1263, Marseille, France

[13] CHU La Concept, AP HM, Dept Haematol & Vasc Biol, Marseille, France

[14] Agcy Sci Technol & Res, Inst Med Biol, Singapore, Singapore

[15] Agcy Sci Technol & Res, Inst Mol & Cell Biol, Singapore, Singapore

[16] Natl Univ Singapore, Yong Loo Lin Sch Med, Dept Surg, Singapore, Singapore

[17] Univ N Carolina, Dept Med, UNC Blood Res Ctr, Chapel Hill, NC USA

[18] Kings Coll London, Sch Cardiovasc Med & Sci, British Heart Fdn Ctr, London, England

[19] Hop Europeen Georges Pompidou, Dept Cardiovasc Surg, Paris, France

[20] Univ Utrecht, Univ Med Ctr Utrecht, UMC Utrecht Regenerat Med Ctr, Lab Expt Cardiol,Dept Cardiol, Utrecht, Netherlands

[21] Univ Utrecht, Univ Med Ctr Utrecht, Circulatory Hlth Lab, Utrecht, Netherlands

[22] Univ Amsterdam, Vesicle Observat Ctr, Amsterdam UMC, Amsterdam, Netherlands

[23] Univ Amsterdam, Lab Expt Clin Chem, Amsterdam UMC, Amsterdam, Netherlands

[24] Icahn Sch Med Mt Sinai, Cardiovasc Res Inst, New York, NY USA

[25] Hannover Med Sch, Inst Mol & Translat Therapeut Strategies, Hannover, Germany

[26] Fraunhofer Inst Toxicol & Expt Med, Hannover, Germany

[27] Univ Med Ctr Utrecht, CDL Res, Heidelberglaan 100, NL-3584 CX Utrecht, Netherlands

[28] Univ Utrecht, Fac Vet Med, Dept Biomol Hlth Sci, Yalelaan 2, Utrecht, Netherlands

[29] Johns Hopkins Univ, Sch Med, Dept Mol & Comparat Pathobiol, Baltimore, MD USA

[30] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA

来源：

CARDIOVASCULAR RESEARCH | 2023年 / 119卷 / 01期

基金：

奥地利科学基金会; 美国国家卫生研究院; 欧盟地平线“2020”; 欧洲研究理事会;

关键词：

Exosomes; Microvesicles; Cardiovascular diseases; Biodistribution; Therapeutics; Blood; Heart; Extracellular vesicle composition; TISSUE FACTOR ACTIVITY; OXIDATION-SPECIFIC EPITOPES; THERAPEUTIC APPLICATIONS; EUROPEAN-SOCIETY; CARDIAC-FUNCTION; FLOW-CYTOMETRY; POSITION PAPER; WORKING GROUP; PLASMA; MICROPARTICLES;

D O I：

10.1093/cvr/cvac031

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communications. Two types of EVs of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA, and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.

引用

页码：45 / 63

页数：19

共 188 条

[81] Electroporation-induced siRNA precipitation obscures the efficiency of siRNA loading into extracellular vesicles [J].

Kooijmans, Sander A. A. ;

Stremersch, Stephan ;

Braeckmans, Kevin ;

de Smedt, Stefaan C. ;

Hendrix, An ;

Wood, Matthew J. A. ;

Schiffelers, Raymond M. ;

Raemdonck, Koen ;

Vader, Pieter .

JOURNAL OF CONTROLLED RELEASE, 2013, 172 (01) :229-238

[82] Secretory Mechanisms and Intercellular Transfer of MicroRNAs in Living Cells [J].

Kosaka, Nobuyoshi ;

Iguchi, Haruhisa ;

Yoshioka, Yusuke ;

Takeshita, Fumitaka ;

Matsuki, Yasushi ;

Ochiya, Takahiro .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (23) :17442-17452

[83] Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes [J].

Kowal, Joanna ;

Arras, Guillaume ;

Colombo, Marina ;

Jouve, Mabel ;

Morath, Jakob Paul ;

Primdal-Bengtson, Bjarke ;

Dingli, Florent ;

Loew, Damarys ;

Tkach, Mercedes ;

Thery, Clotilde .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2016, 113 (08) :E968-E977

[84] Extracellular vesicle species differentially affect endothelial cell functions and differentially respond to exercise training in patients with chronic coronary syndromes [J].

Kraenkel, Nicolle ;

Straessler, Elisabeth ;

Uhlemann, Madlen ;

Mueller, Maja ;

Briand-Schumacher, Sylvie ;

Klingenberg, Roland ;

Schulze, P. Christian ;

Adams, Volker ;

Schuler, Gerhard ;

Luescher, Thomas F. ;

Moebius-Winkler, Sven ;

Landmesser, Ulf .

EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY, 2021, 28 (13) :1467-1474

[85] Standardization of pre-analytical variables in plasma microparticle determination: results of the International Society on Thrombosis and Haemostasis SSC Collaborative workshop [J].

Lacroix, R. ;

Judicone, C. ;

Mooberry, M. ;

Boucekine, M. ;

Key, N. S. ;

Dignat-George, F. .

JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2013, 11 (06) :1190-1193

[86]

Lacroix R., 2019, ISTH SSC VASCULAR BI

[87] Microvesicles and Cancer Associated Thrombosis [J].

Lacroix, Romaric ;

Vallier, Loris ;

Bonifay, Amandine ;

Simoncini, Stephanie ;

Mege, Diane ;

Aubert, Mathilde ;

Panicot-Dubois, Laurence ;

Dubois, Christophe ;

Dignat-George, Francoise .

SEMINARS IN THROMBOSIS AND HEMOSTASIS, 2019, 45 (06) :593-603

[88] Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury [J].

Lai, Ruenn Chai ;

Arslan, Fatih ;

Lee, May May ;

Sze, Newman Siu Kwan ;

Choo, Andre ;

Chen, Tian Sheng ;

Salto-Tellez, Manuel ;

Timmers, Leo ;

Lee, Chuen Neng ;

El Oakley, Reida Menshawe ;

Pasterkamp, Gerard ;

de Kleijn, Dominique P. V. ;

Lim, Sai Kiang .

STEM CELL RESEARCH, 2010, 4 (03) :214-222

[89] Derivation and characterization of human fetal MSCs: An alternative cell source for large-scale production of cardioprotective microparticles [J].

Lai, Ruenn Chai ;

Arslan, Fatih ;

Tan, Soon Sim ;

Tan, Betty ;

Choo, Andre ;

Lee, May May ;

Chen, Tian Sheng ;

Teh, Bao Ju ;

Eng, John Kun Long ;

Sidik, Harwin ;

Tanavde, Vivek ;

Hwang, Wei Sek ;

Lee, Chuen Neng ;

El Oakley, Reida Menshawe ;

Pasterkamp, Gerard ;

de Kleijn, Dominique P. V. ;

Tan, Kok Hian ;

Lim, Sai Kiang .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2010, 48 (06) :1215-1224

[90] Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing [J].

Lasser, Cecilia ;

Shelke, Ganesh Vilas ;

Yeri, Ashish ;

Kim, Dae-Kyum ;

Crescitelli, Rossella ;

Raimondo, Stefania ;

Sjostrand, Margareta ;

Gho, Yong Song ;

Jensen, Kendall Van Keuren ;

Lotvall, Jan .

RNA BIOLOGY, 2017, 14 (01) :58-72

← 4 5 6 7 8 9 10 11 12 13 →