Effects of grape peel phenolics on lipid accumulation in sodium palmitate-treated HepG2 cells

被引：12

作者：

Cui, Wenyu ^{[1
,3
]}

Xu, Ben ^{[1
]}

Chen, Fuchun ^{[1
]}

Shen, Weijun ^{[2
]}

Wan, Fachun ^{[2
]}

Cheng, Anwei ^{[1
,3
]}

机构：

[1] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China

[2] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China

[3] Hunan Agr Univ, Coll Food Sci & Technol, 1 Nongda Rd, Changsha, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2024年 / 112卷

关键词：

Grape peel phenolics; Lipid accumulation; Oxidative stress; AMPK/mTOR/AKT signaling pathways; HepG2; cells; FATTY LIVER-DISEASE; INSULIN-RESISTANCE; HEPATIC STEATOSIS; OXIDATIVE STRESS; EXTRACT; BIOAVAILABILITY; PATHOGENESIS; INFLAMMATION; ANTIOXIDANT; PATHWAY;

D O I：

10.1016/j.jff.2023.105923

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Phenolics were extracted from grape peels, and the compounds of grape peel phenolics (GPP) were identified by LC-MS/MS. From a perspective of dietary nutrition, this study aimed to evaluate the effect of GPP on lipid accumulation in sodium palmitate (PA)-treated HepG2 cells, and further to explore its mechanisms. HepG2 cells were induced by 0.4 mmol/L PA as an in vitro model of lipid accumulation. The results showed that GPP could significantly reduce the TG level and ROS production in PA-treated HepG2 cells. RT-qPCR and Western blotting analysis indicated that GPP supplementation can downregulate the mRNA and protein expression of lipogenesis factors and upregulate the expression of lipolysis factors in PA-treated cells. GPP reduced lipid accumulation and oxidative stress by activating the AMPK/mTOR/AKT signaling pathways, and the most significant effect was detected at 250 mu g/mL GPP. Thus, GPP as a dietary phenolic, have the function to regulate lipid metabolism.

引用

页数：10

共 56 条

[31] Delphinidin Ameliorates Hepatic Triglyceride Accumulation in Human HepG2 Cells, but Not in Diet-Induced Obese Mice [J].

Parra-Vargas, Marcela ;

Sandoval-Rodriguez, Ana ;

Rodriguez-Echevarria, Roberto ;

Dominguez-Rosales, Jose Alfredo ;

Santos-Garcia, Arturo ;

Armendariz-Borunda, Juan .

NUTRIENTS, 2018, 10 (08)

[32] Pretreatment of hydroethanolic extract of Dillenia indica L. attenuates oleic acid induced NAFLD in HepG2 cells via modulating SIRT-1/p-LKB-1/AMPK, HMGCR & PPAR-α signaling pathways [J].

Poornima, M. S. ;

Sindhu, G. ;

Billu, Abraham ;

Sruthi, C. R. ;

Nisha, P. ;

Gogoi, Pinku ;

Baishya, Gakul ;

Raghu, K. G. .

JOURNAL OF ETHNOPHARMACOLOGY, 2022, 292

[33] Dietary Polyphenols Protect Against Oleic Acid-Induced Steatosis in an in Vitro Model of NAFLD by Modulating Lipid Metabolism and Improving Mitochondrial Function [J].

Rafiei, Hossein ;

Omidian, Kosar ;

Bandy, Brian .

NUTRIENTS, 2019, 11 (03)

[34] C-Phycocyanin inhibits hepatic gluconeogenesis and increases glycogen synthesis via activating Akt and AMPK in insulin resistance hepatocytes [J].

Ren, Zhiheng ;

Xie, Zhifei ;

Cao, Danni ;

Gong, Mufeng ;

Yang, Lei ;

Zhou, Zhu ;

Ou, Yu .

FOOD & FUNCTION, 2018, 9 (05) :2829-2839

[35] The antioxidant role of pterostilbene in streptozotocin-nicotinamide-induced type 2 diabetes mellitus in Wistar rats [J].

Satheesh, M. Amarnath ;

Pari, L. .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 2006, 58 (11) :1483-1490

[36]

Saxton RA, 2017, CELL, V168, P960, DOI [10.1016/j.cell.2017.02.004, 10.1016/j.cell.2017.03.035]

[37] Aucubin inhibited lipid accumulation and oxidative stress via Nrf2/HO-1 and AMPK signalling pathways [J].

Shen, Bingyu ;

Zhao, Chenxu ;

Wang, Yue ;

Peng, Yi ;

Cheng, Jiaqi ;

Li, Zheng ;

Wu, Lin ;

Jin, Meiyu ;

Feng, Haihua .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2019, 23 (06) :4063-4075

[38] Quercetin Attenuates Osteoporosis in Orchiectomy Mice by Regulating Glucose and Lipid Metabolism via the GPRC6A/AMPK/mTOR Signaling Pathway [J].

Sun, Jie ;

Pan, Yalan ;

Li, Xiaofeng ;

Wang, Lining ;

Liu, Mengmin ;

Tu, Pengcheng ;

Wu, Chengjie ;

Xiao, Jirimutu ;

Han, Qiuge ;

Da, Weiwei ;

Ma, Yong ;

Guo, Yang .

FRONTIERS IN ENDOCRINOLOGY, 2022, 13

[39] Gallic Acid Inhibits Lipid Accumulation via AMPK Pathway and Suppresses Apoptosis and Macrophage-Mediated Inflammation in Hepatocytes [J].

Tanaka, Miori ;

Sato, Akari ;

Kishimoto, Yoshimi ;

Mabashi-Asazuma, Hideaki ;

Kondo, Kazuo ;

Iida, Kaoruko .

NUTRIENTS, 2020, 12 (05)

[40] Resveratrol supplement inhibited the NF-κB inflammation pathway through activating AMPKα-SIRT1 pathway in mice with fatty liver [J].

Tian, Yueli ;

Ma, Jingting ;

Wang, Wudong ;

Zhang, Lingjuan ;

Xu, Jia ;

Wang, Kai ;

Li, Dongfu .

MOLECULAR AND CELLULAR BIOCHEMISTRY, 2016, 422 (1-2) :75-84

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