Immunohistochemical detection of EGFRvIII in glioblastoma - Anti-EGFRvIII antibody validation for diagnostic and CAR-T purposes

被引：0

作者：

Rutkowska, Adrianna ^{[1
,2
,3
]}

Strozik, Tadeusz ^{[1
]}

Jedrychowska-Danska, Krystyna ^{[1
]}

Zamerska, Alicja ^{[1
]}

Jesionek-Kupnicka, Dorota ^{[4
]}

Kowalczyk, Tamara ^{[4
]}

Och, Waldemar ^{[5
]}

Szostak, Blazej ^{[6
]}

Treda, Cezary ^{[2
,3
,7
]}

Wlodarczyk, Aneta ^{[2
,3
,7
]}

Kierasinska-Kalka, Amelia ^{[2
,3
,7
]}

Wasiak, Tomasz ^{[1
]}

Ciunowicz, Damian ^{[1
,2
,3
]}

Rieske, Piotr ^{[2
,3
,7
]}

Stoczynska-Fidelus, Ewelina ^{[1
,2
,3
]}

机构：

[1] Med Univ Lodz, Dept Mol Biol, Zeligowskiego 7-9, PL-90752 Lodz, Poland

[2] Celther Polska LTD, Dept Res & Dev, Inwestycyjna 7, PL-95050 Konstantynow Lodzki, Poland

[3] Personather LTD, Dept Res & Dev, Inwestycyjna 7, PL-95050 Konstantynow Lodzki, Poland

[4] Med Univ Lodz, Chair Oncol, Dept Pathol, Lodz, Poland

[5] Reg Specialist Hosp Olsztyn, Clin Dept Neurosurg, Zolnierska 18, PL-10561 Olsztyn, Poland

[6] Reg Specialist Hosp Olsztyn, Dept Pathomorphol, Zolnierska 18, PL-10561 Olsztyn, Poland

[7] Med Univ Lodz, Dept Tumor Biol, Zeligowskiego 7-9, PL-90752 Lodz, Poland

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2023年 / 685卷

关键词：

EGFRvIII; CAR-T; Glioblastoma; VARIANT III EGFRVIII; CELL THERAPY; EXPRESSION; GROWTH; HEAD;

D O I：

10.1016/j.bbrc.2023.149133

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The emergence of therapies such as CAR-T has created a need for reliable, validated methods for detecting EGFRvIII in patient tumor cells. Particularly so since previous studies have already suggested that some anti-EGFRvIII antibodies may be non-specific. The present paper evaluates the use of the L8A4 antibody in the immunohistochemical (IHC) and immunocytochemical (ICC) detection of EGFRvIII in 30 glioblastoma speci-mens, and compares it with other methods such as RT-PCR, MLPA, and FISH. The results indicate that Real-time PCR appears to be a very specific and sensitive method of EGFRvIII detection. ICC analysis with L8A4 also appears specific but requires cell culture. IHC analyses of EGFRvIII returned a number of false positives when using L8A4. Due to the growing need for an effective diagnostic tool before starting immunotherapy methods, such as the CAR-T anti-EGFRvIII or SynNotch CAR-T recognizing EGFRvIII, it is necessary to identify a more reliable and simple method of EGFRvIII detection or improve the specificity of the anti-EGFRvIII antibody, until then, immunocytochemistry may temporarily replace immunohistochemistry.

引用

页数：12