Recombinant measles virus encoding the spike protein of SARS-CoV-2 efficiently induces Th1 responses and neutralizing antibodies that block SARS-CoV-2 variants

被引：4

作者：

Kwak, Hye Won ^{[1
,2
,3
]}

Park, Hyo-Jung ^{[1
,2
]}

Jung, Seo-Yeon ^{[4
]}

Oh, Eun Young ^{[5
]}

Park, Sang-In ^{[6
]}

Kim, Yeonhwa ^{[5
]}

Park, Hyeong-Jun ^{[1
,2
,3
]}

Park, Sohyun ^{[5
]}

Kim, You-Jin ^{[7
]}

Ko, Hae Li ^{[6
]}

Lee, Jung-Ah ^{[7
]}

Won, Hyeran ^{[7
]}

Hwang, Yun-Ho ^{[7
]}

Kim, Seo Yeon ^{[7
]}

Kim, Se Eun ^{[7
]}

Bae, Seoung Eun ^{[8
]}

Yoon, Minhyuk ^{[8
]}

Kim, Jae-Ouk ^{[8
]}

Song, Manki ^{[8
]}

Lee, Su Jeen ^{[4
]}

Seo, Ki-Weon ^{[4
]}

Lee, Kunse ^{[4
]}

Kim, Dokeun ^{[7
]}

Kim, Hun ^{[4
]}

Lee, Sang-Myeong ^{[5
]}

Hong, So-Hee ^{[9
]}

Nam, Jae-Hwan ^{[1
,2
,10
]}

机构：

[1] Catholic Univ Korea, Dept Med & Biol Sci, Gyeonggi Do, Bucheon, South Korea

[2] Catholic Univ Korea, BK Plus Dept Biotechnol, Gyeonggi Do, Bucheon, South Korea

[3] SML Biopharm, Gyeonggi Do, Bucheon, South Korea

[4] SK Biosci, Dept R&D, Pangyoro, South Korea

[5] Chungbuk Natl Univ, Coll Vet Med, Cheongju 28644, South Korea

[6] Scripps Korea Antibody Inst, Div Res Program, Chunchon, Kangwon Do, South Korea

[7] Natl Inst Hlth, Natl Inst Infect Dis, Ctr Vaccine Res, Div Infect Dis Vaccine Res, Sejong, South Korea

[8] Int Vaccine Inst, Sci Unit, Seoul 08826, South Korea

[9] Ewha Womans Univ, Coll Med, Dept Microbiol, Seoul 07804, South Korea

[10] Catholic Univ Korea, Dept Med & Biol Sci, 43-1 Yeokgok Dong, Bucheon 14662, South Korea

来源：

VACCINE | 2023年 / 41卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

SARS-CoV-2; Measles virus vector; Neutralizing antibody; VACCINE;

D O I：

10.1016/j.vaccine.2023.02.005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Owing to the rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its vari-ants, the development of effective and safe vaccines has become a priority. The measles virus (MeV) vac-cine is an attractive vaccine platform as it has been administered to children for more than 40 years in over 100 countries. In this study, we developed a recombinant MeV expressing the full-length SARS-CoV-2 spike protein (rMeV-S) and tested its efficacy using mouse and hamster models. In hCD46Tg mice, two-dose rMeV-S vaccination induced higher Th1 secretion and humoral responses than one-dose vacci-nation. Interestingly, neutralizing antibodies induced by one-dose and two-dose rMeV-S immunization effectively blocked the entry of the a, b, c, and d variants of SARS-CoV-2. Furthermore, two-dose rMeV-S immunization provided complete protection against SARS-CoV-2 in the hamster model. These results suggest the potential of rMeV-S as a vaccine candidate for targeting SARS-CoV-2 and its variants.(c) 2023 Elsevier Ltd. All rights reserved.

引用

页码：1892 / 1901

页数：10

共 50 条

[41] The dynamics of antibodies to SARS-CoV-2 in a case of SARS-CoV-2 infection
Xia, Yong
Hong, Honghai
Feng, Yao
Liu, Meiling
Pan, Xingfei
Chen, Dexiong
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2020, 96 : 359 - 360
[42] An unconventional strategy for purifying recombinant SARS-CoV-2 spike protein
Ingawale, Mrunal
Riaz, Mohammad
Durocher, Yves
Ghosh, Raja
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2024, 1247
[43] SARS-CoV-2 variants- Evolution, spike protein, and vaccines
Chen, Kai-Wei K.
Huang, Daniel Tsung-Ning
Huang, Li -Min
BIOMEDICAL JOURNAL, 2022, 45 (04) : 573 - 579
[44] Monoclonal neutralizing antibodies against SARS-COV-2 S protein
Cheng, Lin-Dong
Li, Ping
Lin, Yan-Chen
Hu, Hui-Xiu
Zhang, Ying
Li, Hou-Feng
Huang, Jing
Tan, Li
Ma, Ning
Xia, Deng-Yun
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, 2024, 16 (02): : 681 - 689
[45] Receptor-binding domain of SARS-CoV-2 spike protein efficiently inhibits SARS-CoV-2 infection and attachment to mouse lung
Shin, Hye Jin
Ku, Keun Bon
Kim, Hae Soo
Moon, Hyun Woo
Jeong, Gi Uk
Hwang, Insu
Yoon, Gun Young
Lee, Sunhee
Lee, Sumin
Ahn, Dae-Gyun
Kim, Kyun-Do
Kwon, Young-Chan
Kim, Bum-Tae
Kim, Seong-Jun
Kim, Chonsaeng
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES, 2021, 17 (14): : 3786 - 3794
[46] An Analysis of the Neutralizing Antibodies against the Main SARS-CoV-2 Variants in Healthcare Workers (HCWs) Vaccinated against or Infected by SARS-CoV-2
Immordino, Palmira
Pisciotta, Vincenzo
Amodio, Emanuele
Bonura, Celestino
Bonura, Floriana
Cacioppo, Federica
Calamusa, Giuseppe
Capra, Giuseppina
Casuccio, Alessandra
De Grazia, Simona
Genovese, Dario
Graci, Davide
Lacca, Guido
Sanfilippo, Giuseppa Luisa
Verso, Maria Gabriella
Giammanco, Giovanni Maurizio
Ferraro, Donatella
VACCINES, 2023, 11 (11)
[47] Serosurvey in SARS-CoV-2 inactivated vaccine-elicited neutralizing antibodies against authentic SARS-CoV-2 and its viral variants
Zou, Lirong
Zhang, Huan
Zheng, Zhonghua
Jiang, Yushan
Huang, Yushi
Lin, Shujian
Yu, Jianxiang
Deng, Xiaoling
He, Jianfeng
Shen, Chenguang
Li, Baisheng
JOURNAL OF MEDICAL VIROLOGY, 2022, 94 (12) : 6065 - 6072
[48] Intranasal immunization with recombinant Vaccinia virus encoding trimeric SARS-CoV-2 spike receptor-binding domain induces neutralizing antibody
Cao, Xiaoling
Zai, Junjie
Zhao, Qingzhen
Xie, Lilan
Li, Yaoming
VACCINE, 2022, 40 (40) : 5757 - 5763
[49] Comparison of the SARS-CoV-2 spike protein ELISA and the Abbott Architect SARS-CoV-2 IgG nucleocapsid protein assays for detection of antibodies
Wadhwa, Ashutosh
Yin, Sherry
Freeman, Brandi
Hershow, Rebecca B.
Killerby, Marie
Yousaf, Anna R.
Lester, Sandra
Mills, Lisa
Buono, Sean A.
Pomeroy, Mary
Owusu, Daniel
Chu, Victoria T.
Tate, Jacqueline E.
Bhattacharyya, Sanjib
Hall, Patricia
Thornburg, Natalie J.
Kirking, Hannah L.
PLOS ONE, 2021, 16 (07):
[50] Comprehensive Overview of Broadly Neutralizing Antibodies against SARS-CoV-2 Variants
Cui, Lingyan
Li, Tingting
Xue, Wenhui
Zhang, Sibo
Wang, Hong
Liu, Hongjing
Gu, Ying
Xia, Ningshao
Li, Shaowei
VIRUSES-BASEL, 2024, 16 (06):

← 1 2 3 4 5 →